Mouse Nucleobindin 1 (NUCB1) ELISA Kit | NUCB1 elisa kit

Mouse Nucleobindin 1 (NUCB1) ELISA Kit

Cat. Num. AAA2022420

• Gene Names: NUCB1; NUC; CALNUC
• Reactivity: Mouse
• Synonyms: Nucleobindin 1 (NUCB1); Mouse Nucleobindin 1 (NUCB1) ELISA Kit; NUC; CALNUC; NUCB1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nucleobindin 1 (NUCB1).; No significant cross-reactivity or interference between Nucleobindin 1 (NUCB1) and analogues was observed.
• Sequence Length: 461

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleobindin 1 (NUCB1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleobindin 1 (NUCB1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NUCB1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleobindin 1 (NUCB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleobindin 1 (NUCB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleobindin 1 (NUCB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nucleobindin 1 (NUCB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NUCB1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 12803105
• NCBI GeneID: 4924
• UniProt Accession #: Q02818; Q15838; Q7Z4J7; Q9BUR1; B2RD64
• Molecular Weight: 53,879 Da
• NCBI Official Full Name: Nucleobindin 1
• NCBI Official Synonym Full Names: nucleobindin 1
• NCBI Official Symbol: NUCB1
• NCBI Official Synonym Symbols: NUC; CALNUC
• NCBI Protein Information: nucleobindin-1
• UniProt Protein Name: Nucleobindin-1
• Protein Family: Nucleobindin
• UniProt Gene Name: NUCB1
• UniProt Synonym Gene Names: NUC
• UniProt Entry Name: NUCB1_HUMAN

NCBI Description

This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq, Jun 2010]

Uniprot Description

NUCB1: Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis. Belongs to the nucleobindin family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: microtubule cytoskeleton; Golgi apparatus; extracellular space; membrane; ER-Golgi intermediate compartment

Molecular Function: protein binding; DNA binding; calcium ion binding