Mouse NGF/NGF beta ELISA Kit | NGF elisa kit

Mouse NGF/NGF beta PicoKine ELISA Kit

Cat. Num. AAA175827

• Gene Names: Ngf; Ngfb
• Reactivity: Mouse
• Synonyms: NGF/NGF beta; Mouse NGF/NGF beta PicoKine ELISA Kit; Beta-nerve growth factor; nerve growth factor (beta polypeptide); Beta nerve growth factor; Beta NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor (beta polypeptide); Nerve growth factor beta polypeptide; Nerve growth factor beta subunit; NGFB; NID67; NGF elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Ngf
• Sequence Length: 241

• Samples: Cell culture supernatants, serum and plasma (heparin, edta, citrate)
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• Sensitivity: <1 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NGF elisa kit

Principle of the Assay: The Picokine™ Mouse Ngf Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Ngf with a 96-well strip plate that is pre-coated with antibody specific for Ngf. The detection antibody is a biotinylated antibody specific for Ngf. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Ngf with immunogen: Expression system for standard: NS0; Immunogen sequence: S122-G241. The kit is analytically validated with ready-to-use reagents. To measure Mouse Ngf, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Ngf in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Ngf in the sample.

Background/Introduction: Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.

NCBI and Uniprot Product Information

• NCBI GI #: 128162
• NCBI GeneID: 18049
• UniProt Accession #: P01139; Q63864; Q6LDB7
• Molecular Weight: 27,077 Da
• NCBI Official Full Name: Beta-nerve growth factor
• NCBI Official Synonym Full Names: nerve growth factor
• NCBI Official Symbol: Ngf
• NCBI Official Synonym Symbols: Ngfb
• NCBI Protein Information: beta-nerve growth factor; beta-NGF; nerve growth factor, beta
• UniProt Protein Name: Beta-nerve growth factor
• Protein Family: Nerve growth factor
• UniProt Gene Name: Ngf
• UniProt Synonym Gene Names: Ngfb; Beta-NGF
• UniProt Entry Name: NGF_MOUSE

Uniprot Description

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cytoplasmic membrane-bound vesicle; extracellular region

Molecular Function: metalloendopeptidase inhibitor activity; nerve growth factor receptor binding; growth factor activity; receptor signaling protein activity; receptor binding

Biological Process: positive regulation of neuron maturation; regulation of neuron differentiation; adult locomotory behavior; positive regulation of axon extension; regulation of neurotransmitter secretion; sensory perception of pain; positive regulation of cell growth; positive regulation of protein amino acid autophosphorylation; positive regulation of nerve growth factor receptor signaling pathway; memory; regulation of release of sequestered calcium ion into cytosol; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; positive regulation of cell proliferation; positive regulation of transcription factor activity; negative regulation of neuron apoptosis; positive regulation of neuron differentiation; positive regulation of collateral sprouting; transmembrane receptor protein tyrosine kinase signaling pathway; neurite morphogenesis; neurite development