Mouse Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit | NGAL elisa kit

Mouse Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit

Cat. Num. AAA453267

• Gene Names: Lcn2; NRL; 24p3; Sip24; AW212229
• Reactivity: Mouse
• Synonyms: Neutrophil Gelatinase Associated Lipocalin (NGAL); Mouse Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit; NGAL elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of NGAL. No significant cross-reactivity or interference between NGAL and analogues was observed.
• Sequence Length: 200

• Samples: Serum, plasma, tissue homogenates, urine, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.285ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NGAL were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NGAL were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NGAL elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NGAL in mouse serum, plasma, tissue homogenates, urine, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGAL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NGAL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NGAL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NGAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 34328049
• NCBI GeneID: 16819
• NCBI Accession #: NP_032517.1
• NCBI GenBank Nucleotide #: NM_008491.1
• UniProt Accession #: P11672; Q3UE34
• Molecular Weight: 22,875 Da
• NCBI Official Full Name: neutrophil gelatinase-associated lipocalin
• NCBI Official Synonym Full Names: lipocalin 2
• NCBI Official Symbol: Lcn2
• NCBI Official Synonym Symbols: NRL; 24p3; Sip24; AW212229
• NCBI Protein Information: neutrophil gelatinase-associated lipocalin
• UniProt Protein Name: Neutrophil gelatinase-associated lipocalin
• UniProt Gene Name: Lcn2
• UniProt Synonym Gene Names: NGAL

Uniprot Description

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.