TFF1 recombinant protein

TFF1

Cat. Num. AAA517724

• Gene Names: TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
• Applications: Cell Assay
• Synonyms: TFF1; Breast cancer estrogen-inducible protein; BCEI; PNR-2; Polypeptide P1.A; Protein pS2; Recombinant Human Trefoil Factor 1 (TFF1); TFF1 recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS

• Applicable Applications for TFF1 recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method.
• Sample Cell Assay Data: The ED (50) was determined by the dose-dependent proliferation of MCF7 cells and was found to be <0.5ng/mL.
• Reconstitution: Reconstitute at 100ug/mL in sterile PBS
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

SDS-Page

(Purity: Recombinant Human TFF1 resolved on a 17% SDS_PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250)

Related Product Information for TFF1 recombinant protein

Recombinant Human TFF1 was expressed in E Coli cells. The protein accession number is P04155
Found in the gastrointestinal tract, the three Trefoil proteins, TFF1, 2, and 3 have been shown to play an important role in intestinal mucosal defence and repair. Specifically, TFF1 is essential for normal differentiation of the antral and pyloric gastric mucosa and functions as a gastric-specific tumor suppressor gene.

Product Categories/Family for TFF1 recombinant protein

Growth Factors; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4507451
• NCBI GeneID: 7031
• NCBI Accession #: NP_003216.1
• NCBI GenBank Nucleotide #: NM_003225.2
• UniProt Accession #: P04155
• Molecular Weight: 9,150 Da
• NCBI Official Full Name: trefoil factor 1
• NCBI Official Synonym Full Names: trefoil factor 1
• NCBI Official Symbol: TFF1
• NCBI Official Synonym Symbols: pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
• NCBI Protein Information: trefoil factor 1; protein pS2; polypeptide P1.A; gastrointestinal trefoil protein pS2; breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence
• UniProt Protein Name: Trefoil factor 1
• Protein Family: Trefoil factor
• UniProt Gene Name: TFF1
• UniProt Synonym Gene Names: BCEI; PS2; hP1.A
• UniProt Entry Name: TFF1_HUMAN

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

TFF1: Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular space

Molecular Function: protein binding; growth factor activity

Biological Process: negative regulation of cell proliferation; response to peptide hormone stimulus; maintenance of gastrointestinal epithelium; digestion; carbohydrate metabolic process; cell differentiation; response to iron ion; response to estradiol stimulus