Mouse Nanog homeobox ELISA Kit | Nanog elisa kit

Mouse NANOG Homeobox protein, NANOG ELISA Kit

Cat. Num. AAA2602965

• Gene Names: Nanog; ENK; ecat4; 2410002E02Rik
• Reactivity: Mouse
• Synonyms: Nanog homeobox; Mouse NANOG Homeobox protein; NANOG ELISA Kit; Nanog elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.

• Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant NANOG concentration
• Assay Type: Sandwich
• Detection Range: 100 ng/ml-1.56 ng/ml
• Sensitivity: 0.5 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Nanog elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse NANOG monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 577019513
• NCBI GeneID: 71950
• NCBI Accession #: NP_001276757.1
• NCBI GenBank Nucleotide #: NM_001289828.1
• UniProt Accession #: Q80Z64; Q6SMR1; Q7TN85; Q5TM83
• Molecular Weight: 34,240 Da
• NCBI Official Full Name: homeobox protein NANOG isoform 2
• NCBI Official Synonym Full Names: Nanog homeobox
• NCBI Official Symbol: Nanog
• NCBI Official Synonym Symbols: ENK; ecat4; 2410002E02Rik
• NCBI Protein Information: homeobox protein NANOG; ES cell-associated protein 4; homeobox transcription factor Nanog; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein
• UniProt Protein Name: Homeobox protein NANOG
• Protein Family: Homeobox protein
• UniProt Gene Name: Nanog
• UniProt Synonym Gene Names: Ecat4; Enk
• UniProt Entry Name: NANOG_MOUSE

NCBI Description

The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

NANOG: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. Interacts with SMAD1 and SALL4. Expressed in testicular carcinoma and derived germ cell tumors. Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Belongs to the Nanog homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Transcription factor; DNA-binding

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; chromatin binding; transcription corepressor activity; transcription factor activity

Biological Process: response to retinoic acid; transcription, DNA-dependent; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; multicellular organismal development; stem cell division; endodermal cell fate specification; stem cell maintenance; stem cell differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; mesodermal cell fate commitment; negative regulation of BMP signaling pathway; negative regulation of cell fate commitment; response to organic substance; cell proliferation; embryonic development; negative regulation of cell differentiation; regulation of transcription, DNA-dependent; regulation of gene expression; regulation of cell differentiation; positive regulation of cell proliferation; negative regulation of endodermal cell fate specification; positive regulation of transcription from RNA polymerase II promoter