Rabbit anti-Human PSRC1 Polyclonal Antibody | anti-PSRC1 antibody

PSRC1, CT (PSRC1, DDA3, Proline/serine-rich coiled-coil protein 1) (Azide free) (HRP)

Cat. Num. AAA6338385

• Gene Names: PSRC1; DDA3; FP3214
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PSRC1; Polyclonal Antibody; CT (PSRC1; DDA3; Proline/serine-rich coiled-coil protein 1) (Azide free) (HRP); Proline/serine-rich coiled-coil protein 1; anti-PSRC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PSRC1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PSRC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343-371 amino acids from the C-terminal region of human PSRC1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry (FC/FACS)

(PSRC1 Antibody (C-Term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PSRC1 antibody

This gene encodes a proline-rich protein. Studies of this gene and the related mouse gene suggest that this gene is regulated by p53 and may participate in p53-mediated growth suppression. The encoded protein may function as a microtubule destabilizing protein that controls spindle dynamics and mitotic progression by recruiting and regulating microtubule depolymerases. At least one genetic variation in this gene has been associated with decreased serum levels of low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding different isoforms have been described.

Product Categories/Family for anti-PSRC1 antibody

Antibodies; CCDC (Coiled-coil Domain-containing)

References

Hu, M., et al. Pharmacogenet. Genomics 20(10):634-637(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Roder, C., et al. Childs Nerv Syst (2010). In press: Keebler, M.E., et al. Circ Cardiovasc Genet 3(4):358-364(2010). Hsieh, W.J., et al. Biochem. Biophys. Res. Commun. 369(2):567-572(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 73858561
• NCBI GeneID: 84722
• NCBI Accession #: NP_001027462.1; NP_116025.1; NP_001005290.1
• NCBI GenBank Nucleotide #: NM_001032291.2
• UniProt Accession #: Q6PGN9; Q5T2Z3; Q6ZTI8; Q71MG3; Q9BV77
• Molecular Weight: 35,632 Da
• NCBI Official Full Name: proline/serine-rich coiled-coil protein 1 isoform a
• NCBI Official Synonym Full Names: proline and serine rich coiled-coil 1
• NCBI Official Symbol: PSRC1
• NCBI Official Synonym Symbols: DDA3; FP3214
• NCBI Protein Information: proline/serine-rich coiled-coil protein 1
• UniProt Protein Name: Proline/serine-rich coiled-coil protein 1
• Protein Family: Proline/serine-rich coiled-coil protein
• UniProt Gene Name: PSRC1
• UniProt Synonym Gene Names: DDA3

NCBI Description

This gene encodes a proline-rich protein that is a target for regulation by the tumor suppressor protein p53. The encoded protein plays an important role in mitosis by recruiting and regulating microtubule depolymerases that destabalize microtubules. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Apr 2014]

Uniprot Description

Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate, and for normal rate of chromosomal segregation during anaphase. Plays a role in the regulation of mitotic spindle dynamics. Increases the rate of turnover of microtubules on metaphase spindles, and contributes to the generation of normal tension across sister kinetochores. Recruits KIF2A and ANKRD53 to the mitotic spindle and spindle poles. May participate in p53/TP53-regulated growth suppression.