Mouse NANOG Homeobox Protein (NANOG) ELISA Kit | NANOG elisa kit

Mouse NANOG Homeobox Protein (NANOG) ELISA Kit

Cat. Num. AAA9717438

• Gene Names: Nanog; ENK; ecat4; 2410002E02Rik
• Reactivity: Mouse
• Synonyms: NANOG Homeobox Protein (NANOG); Mouse NANOG Homeobox Protein (NANOG) ELISA Kit; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; NANOG elisa kit

• Reactivity: Mouse
• Specificity: Mouse NANOG Homeobox protein (NANOG) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NANOG. No significant cross-reactivity or interference between Mouse NANOG and analogues was observed.
• Sequence Length: 304

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse NANOG Homeobox protein (NANOG) Standard Curve.)

Related Product Information for NANOG elisa kit

Description: This Mouse NANOG Homeobox protein (NANOG) ELISA Kit employs a two-site sandwich ELISA to quantitate NANOG in samples. An antibody specific for NANOG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NANOG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NANOG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NANOG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The Nanog homeobox encoded by Nanog is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Several transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 577019513
• NCBI GeneID: 71950
• NCBI Accession #: NP_001276757.1
• NCBI GenBank Nucleotide #: NP_001276757.1
• UniProt Accession #: Q80Z64; Q5TM83
• NCBI Official Full Name: homeobox protein NANOG isoform 2
• NCBI Official Synonym Full Names: Nanog homeobox
• NCBI Official Symbol: Nanog
• NCBI Official Synonym Symbols: ENK; ecat4; 2410002E02Rik
• NCBI Protein Information: homeobox protein NANOG
• UniProt Protein Name: Homeobox protein NANOG
• Protein Family: Homeobox protein
• UniProt Gene Name: Nanog
• UniProt Synonym Gene Names: Ecat4; Enk
• UniProt Entry Name: NANOG_MOUSE

NCBI Description

The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]