Mouse Myotrophin (MTPN) ELISA Kit | MTPN elisa kit

Mouse Myotrophin (MTPN) ELISA Kit

Cat. Num. AAA2022528

• Gene Names: MTPN; V-1; GCDP
• Reactivity: Mouse
• Synonyms: Myotrophin (MTPN); Mouse Myotrophin (MTPN) ELISA Kit; GCDP; V-1; Granule Cell Differentiation Protein; MTPN elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myotrophin (MTPN).; No significant cross-reactivity or interference between Myotrophin (MTPN) and analogues was observed.
• Sequence Length: 118

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 6.25-400pg/mL
• Sensitivity: < 2.67pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myotrophin (MTPN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myotrophin (MTPN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MTPN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myotrophin (MTPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myotrophin (MTPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myotrophin (MTPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myotrophin (MTPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MTPN elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 21956645
• NCBI GeneID: 136319
• NCBI Accession #: NP_665807.1
• NCBI GenBank Nucleotide #: NM_145808.3
• UniProt Accession #: P58546
• Molecular Weight: 12,895 Da
• NCBI Official Full Name: myotrophin
• NCBI Official Synonym Full Names: myotrophin
• NCBI Official Symbol: MTPN
• NCBI Official Synonym Symbols: V-1; GCDP
• NCBI Protein Information: myotrophin
• UniProt Protein Name: Myotrophin
• Protein Family: Myotrophin
• UniProt Gene Name: MTPN
• UniProt Entry Name: MTPN_HUMAN

NCBI Description

The transcript produced from this gene is bi-cistronic and can encode both myotrophin and leucine zipper protein 6. The myotrophin protein is associated with cardiac hypertrophy, where it is involved in the conversion of NFkappa B p50-p65 heterodimers to p50-p50 and p65-p65 homodimers. This protein also has a potential function in cerebellar morphogenesis, and it may be involved in the differentiation of cerebellar neurons, particularly of granule cells. A cryptic ORF at the 3' end of this transcript uses a novel internal ribosome entry site and a non-AUG translation initiation codon to produce leucine zipper protein 6, a 6.4 kDa tumor antigen that is associated with myeloproliferative disease. [provided by RefSeq, Jul 2008]

Uniprot Description

MTPN: Promotes dimerization of NF-kappa-B subunits and regulates NF-kappa-B transcription factor activity. Plays a role in the regulation of the growth of actin filaments. Inhibits the activity of the F-actin-capping protein complex formed by the CAPZA1 and CAPZB heterodimer. Promotes growth of cardiomyocytes, but not cardiomyocyte proliferation. Promotes cardiac muscle hypertrophy. Belongs to the myotrophin family.

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: F-actin capping protein complex; axon; perinuclear region of cytoplasm; cytosol; nucleus

Biological Process: neuron differentiation; regulation of translation; striated muscle cell differentiation; skeletal muscle regeneration; catecholamine metabolic process; cell growth; positive regulation of protein metabolic process; cerebellar granule cell differentiation; positive regulation of cell growth; regulation of striated muscle development; activation of NF-kappaB transcription factor