Mouse Mothers Against Decapentaplegic Homolog 2 (Smad2) ELISA Kit | Smad2 elisa kit

Mouse Mothers Against Decapentaplegic Homolog 2 (Smad2) ELISA Kit

Cat. Num. AAA2707565

• Gene Names: Smad2; Madh2; Madr2; mMad2; Smad-2; 7120426M23Rik
• Reactivity: Mouse
• Synonyms: Mothers Against Decapentaplegic Homolog 2 (Smad2); Mouse Mothers Against Decapentaplegic Homolog 2 (Smad2) ELISA Kit; JV18; JV18-1; MADH2; MADR2; HMAD-2; HSMAD2; Mad-related protein 2; Smad2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mothers Against Decapentaplegic Homolog 2 (Smad2).; No significant cross-reactivity or interference between Mothers Against Decapentaplegic Homolog 2 (Smad2) and analogues was observed.
• Sequence Length: 467

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 62.5-4,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 28.4pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 2 (Smad2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 2 (Smad2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Smad2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 2 (Smad2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mothers Against Decapentaplegic Homolog 2 (Smad2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mothers Against Decapentaplegic Homolog 2 (Smad2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mothers Against Decapentaplegic Homolog 2 (Smad2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 357197177
• NCBI GeneID: 17126
• NCBI Accession #: NP_001239410.1
• NCBI GenBank Nucleotide #: NP_001239410.1
• UniProt Accession #: Q62432
• NCBI Official Full Name: mothers against decapentaplegic homolog 2 isoform 1
• NCBI Official Synonym Full Names: SMAD family member 2
• NCBI Official Symbol: Smad2
• NCBI Official Synonym Symbols: Madh2; Madr2; mMad2; Smad-2; 7120426M23Rik
• NCBI Protein Information: mothers against decapentaplegic homolog 2
• UniProt Protein Name: Mothers against decapentaplegic homolog 2
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: Smad2
• UniProt Synonym Gene Names: Madh2; Madr2; MAD homolog 2; Mothers against DPP homolog 2; mMad2; SMAD 2; Smad2

Uniprot Description

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator ().