Porcine ATP Binding Cassette Transporter G1 ELISA Kit | ABCG1 elisa kit

Porcine ATP Binding Cassette Transporter G1 ELISA Kit

Cat. Num. AAA737516

• Gene Names: ABCG1; ABC8; WHITE1
• Reactivity: Porcine
• Synonyms: ATP Binding Cassette Transporter G1; Porcine ATP Binding Cassette Transporter G1 ELISA Kit; ABCG1 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This ABCG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine ABCG1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCG1 elisa kit

Principle of the Assay: ABCG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ABCG1 antibody and an ABCG1-HRP conjugate. The assay sample and buffer are incubated together with ABCG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ABCG1 concentration since ABCG1 from samples and ABCG1-HRP conjugate compete for the anti-ABCG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ABCG1 from the sample, fewer sites are left to bind ABCG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCG1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ABCG1 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 16304311
• NCBI GeneID: 9619
• Molecular Weight: 86,628 Da
• NCBI Official Full Name: ATP-binding cassette transporter G1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family G (WHITE), member 1
• NCBI Official Symbol: ABCG1
• NCBI Official Synonym Symbols: ABC8; WHITE1
• NCBI Protein Information: ATP-binding cassette sub-family G member 1; ABC transporter 8; homolog of Drosophila white; ATP-binding cassette transporter 8; ATP-binding cassette transporter member 1 of subfamily G; white protein homolog (ATP-binding cassette transporter 8)
• UniProt Protein Name: ATP-binding cassette sub-family G member 1
• Protein Family: ABC transporter G family
• UniProt Gene Name: ABCG1
• UniProt Synonym Gene Names: ABC8; WHT1
• UniProt Entry Name: ABCG1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCG1: Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, ABC family; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: Golgi membrane; nucleoplasm; Golgi apparatus; recycling endosome; endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrion; integral to plasma membrane; plasma membrane; endosome; external side of plasma membrane

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; phospholipid transporter activity; protein heterodimerization activity; cholesterol transporter activity; cholesterol binding; toxin transporter activity; sterol-transporting ATPase activity; phospholipid binding; ADP binding; ATP binding

Biological Process: cholesterol metabolic process; detection of hormone stimulus; response to high density lipoprotein stimulus; positive regulation of cholesterol biosynthetic process; cholesterol efflux; lipoprotein metabolic process; intracellular cholesterol transport; response to lipid; response to organic substance; amyloid precursor protein catabolic process; cholesterol homeostasis; reverse cholesterol transport; phospholipid efflux; regulation of transcription, DNA-dependent; transmembrane transport; phospholipid homeostasis