Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mitochondrial import receptor subunit TOM34 (TOMM34) Standard Curve.)
Mouse Mitochondrial Import Receptor Subunit TOM34 (TOMM34) ELISA Kit | TOMM34 elisa kit
Mouse Mitochondrial Import Receptor Subunit TOM34 (TOMM34) ELISA Kit
Gene Names
Tomm34; TOM34; 2610100K07Rik
Reactivity
Mouse
Synonyms
Mitochondrial Import Receptor Subunit TOM34 (TOMM34); Mouse Mitochondrial Import Receptor Subunit TOM34 (TOMM34) ELISA Kit; HTOM34P; TOM34; URCC3; mitochondrial import receptor subunit TOM34; outer mitochondrial membrane translocase (34kD); TOMM34 elisa kit
Reactivity
Mouse
Specificity
Mouse Mitochondrial import receptor subunit TOM34 (TOMM34) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TOMM34. No significant cross-reactivity or interference between Mouse TOMM34 and analogues was observed.
Sequence Length
309
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mitochondrial import receptor subunit TOM34 (TOMM34) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mitochondrial import receptor subunit TOM34 (TOMM34) Standard Curve.)
Related Product Information for TOMM34 elisa kit
Description: This Mouse Mitochondrial import receptor subunit TOM34 (TOMM34) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM34 in samples. An antibody specific for TOMM34 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TOMM34 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM34 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM34 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TOMM34 is involved in the import of precursor proteins into mitochondria. The encoded protein has a chaperone-like activity, binding the mature portion of unfolded proteins and aiding their import into mitochondria. This protein, which is found in the cytoplasm and sometimes associated with the outer mitochondrial membrane, has a weak ATPase activity and contains 6 TPR repeats. hTom34 is displayed on the surface of mitochondria and is resistant to extraction under alkaline conditions. Antibodies raised against hTom34 specifically inhibit in vitro import of the mitochondrial precursor protein preornithine transcarbamylase into mitochondria isolated from rat liver. Based on trypsin digestion experiments, the receptor has a large (27 kDa) C-terminal domain exposed to the cytosol.
Background: TOMM34 is involved in the import of precursor proteins into mitochondria. The encoded protein has a chaperone-like activity, binding the mature portion of unfolded proteins and aiding their import into mitochondria. This protein, which is found in the cytoplasm and sometimes associated with the outer mitochondrial membrane, has a weak ATPase activity and contains 6 TPR repeats. hTom34 is displayed on the surface of mitochondria and is resistant to extraction under alkaline conditions. Antibodies raised against hTom34 specifically inhibit in vitro import of the mitochondrial precursor protein preornithine transcarbamylase into mitochondria isolated from rat liver. Based on trypsin digestion experiments, the receptor has a large (27 kDa) C-terminal domain exposed to the cytosol.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mitochondrial import receptor subunit TOM34 isoform a
NCBI Official Synonym Full Names
translocase of outer mitochondrial membrane 34
NCBI Official Symbol
Tomm34
NCBI Official Synonym Symbols
TOM34; 2610100K07Rik
NCBI Protein Information
mitochondrial import receptor subunit TOM34
UniProt Protein Name
Mitochondrial import receptor subunit TOM34
Protein Family
UniProt Gene Name
Tomm34
UniProt Entry Name
TOM34_MOUSE
Uniprot Description
TOMM34: Plays a role in the import of cytosolically synthesized preproteins into mitochondria. Binds the mature portion of precursor proteins. Interacts with cellular components, and possesses weak ATPase activity. May be a chaperone-like protein that helps to keep newly synthesized precursors in an unfolded import compatible state. Belongs to the Tom34 family.
Protein type: Mitochondrial; Chaperone
Cellular Component: nucleoplasm; mitochondrial outer membrane; membrane; mitochondrion; cytoplasm; nucleus
Molecular Function: protein binding; heat shock protein binding
Biological Process: protein targeting to mitochondrion
Similar Products
Product Notes
The Mouse TOMM34 tomm34 (Catalog #AAA9716685) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716685 ELISA Kit recognizes Mouse TOMM34. It is sometimes possible for the material contained within the vial of "Mitochondrial Import Receptor Subunit TOM34 (TOMM34), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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