Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitochondrial import receptor subunit TOM40 homolog (TOMM40) Standard Curve.)
Human Mitochondrial Import Receptor Subunit TOM40 Homolog (TOMM40) ELISA Kit | TOMM40 elisa kit
Human Mitochondrial Import Receptor Subunit TOM40 Homolog (TOMM40) ELISA Kit
Gene Names
TOMM40; TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E
Reactivity
Human
Synonyms
Mitochondrial Import Receptor Subunit TOM40 Homolog (TOMM40); Human Mitochondrial Import Receptor Subunit TOM40 Homolog (TOMM40) ELISA Kit; TOMM40; C19orf1; D19S1177E; PER-EC1; PEREC1; TOM40; mitochondrial outer membrane protein; translocase of outer mitochondrial membrane 40; TOMM40 elisa kit
Reactivity
Human
Specificity
Human Mitochondrial import receptor subunit TOM40 homolog (TOMM40) ELISA Kit has high sensitivity and excellent specificity for detection of Human TOMM40. No significant cross-reactivity or interference between Human TOMM40 and analogues was observed.
Sequence Length
361
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitochondrial import receptor subunit TOM40 homolog (TOMM40) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitochondrial import receptor subunit TOM40 homolog (TOMM40) Standard Curve.)
Related Product Information for TOMM40 elisa kit
Description: This Human Mitochondrial import receptor subunit TOM40 homolog (TOMM40) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM40 in samples. An antibody specific for TOMM40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TOMM40 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM40 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM40 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TOMM40 is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for protein import into mitochondria.The deduced protein contains 361 amino acids. Fractionation of rat liver detected Tomm40 within the mitochondrial fraction. Extraction and antibody-accessibility studies suggested that Tomm40 is embedded within the mitochondrial membrane.Tomm40 was associated with a 400-kD complex containing the mitochondrial import receptors Tom20 and Tom22 and several smaller components. Mitochondria-targeted preproteins interacted with the Tomm40-containing complex during their transit to the mitochondrial matrix, and immunodepletion of Tomm40 caused the loss of preprotein import activity.
Background: TOMM40 is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for protein import into mitochondria.The deduced protein contains 361 amino acids. Fractionation of rat liver detected Tomm40 within the mitochondrial fraction. Extraction and antibody-accessibility studies suggested that Tomm40 is embedded within the mitochondrial membrane.Tomm40 was associated with a 400-kD complex containing the mitochondrial import receptors Tom20 and Tom22 and several smaller components. Mitochondria-targeted preproteins interacted with the Tomm40-containing complex during their transit to the mitochondrial matrix, and immunodepletion of Tomm40 caused the loss of preprotein import activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Mitochondrial import receptor subunit TOM40 homolog
NCBI Official Synonym Full Names
translocase of outer mitochondrial membrane 40
NCBI Official Symbol
TOMM40
NCBI Official Synonym Symbols
TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E
NCBI Protein Information
mitochondrial import receptor subunit TOM40 homolog
UniProt Protein Name
Mitochondrial import receptor subunit TOM40 homolog
Protein Family
UniProt Gene Name
TOMM40
UniProt Synonym Gene Names
C19orf1; PEREC1; TOM40
UniProt Entry Name
TOM40_HUMAN
NCBI Description
The protein encoded by this gene is localized in the outer membrane of the mitochondria. It is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for import of protein precursors into mitochondria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2015]
Uniprot Description
TOMM40: Channel-forming protein essential for import of protein precursors into mitochondria. Belongs to the Tom40 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Mitochondrial
Chromosomal Location of Human Ortholog: 19q13
Cellular Component: nucleoplasm; pore complex; mitochondrial outer membrane translocase complex; mitochondrion; cytoplasm; integral to membrane; integral to mitochondrial outer membrane
Molecular Function: protein transmembrane transporter activity; porin activity
Biological Process: cellular protein metabolic process; ion transport; protein targeting to mitochondrion
Research Articles on TOMM40
Similar Products
Product Notes
The Human TOMM40 tomm40 (Catalog #AAA9713831) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713831 ELISA Kit recognizes Human TOMM40. It is sometimes possible for the material contained within the vial of "Mitochondrial Import Receptor Subunit TOM40 Homolog (TOMM40), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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