Mouse Microsomal Triglyceride Transfer Protein (MTTP) ELISA Kit | MTTP elisa kit

Mouse Microsomal Triglyceride Transfer Protein (MTTP) ELISA Kit

Cat. Num. AAA2023118

• Gene Names: MTTP; ABL; MTP
• Reactivity: Mouse
• Synonyms: Microsomal Triglyceride Transfer Protein (MTTP); Mouse Microsomal Triglyceride Transfer Protein (MTTP) ELISA Kit; MTP; ABL; Microsomal triglyceride transfer protein large subunit; MTTP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of MTTP. No significant cross-reactivity or interference between MTTP and analogues was observed.
• Sequence Length: 20

• Samples: Mouse tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MTTP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MTTP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MTTP elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MTTP in mouse tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to MTTP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to MTTP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MTTP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MTTP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MTTP elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 4261846
• NCBI GeneID: 4547
• Molecular Weight: 2,268 Da
• NCBI Official Full Name: microsomal triglyceride transfer protein, partial
• NCBI Official Synonym Full Names: microsomal triglyceride transfer protein
• NCBI Official Symbol: MTTP
• NCBI Official Synonym Symbols: ABL; MTP
• NCBI Protein Information: microsomal triglyceride transfer protein large subunit
• UniProt Protein Name: Microsomal triglyceride transfer protein
• Protein Family: Microsomal triglyceride transfer protein
• UniProt Gene Name: MTP
• UniProt Entry Name: Q16240_HUMAN

NCBI Description

MTP encodes the large subunit of the heterodimeric microsomal triglyceride transfer protein. Protein disulfide isomerase (PDI) completes the heterodimeric microsomal triglyceride transfer protein, which has been shown to play a central role in lipoprotein assembly. Mutations in MTP can cause abetalipoproteinemia. [provided by RefSeq, Jul 2008]

Uniprot Description

MTTP: Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B. Defects in MTTP are the cause of abetalipoproteinemia (ABL). ABL is an autosomal recessive disorder of lipoprotein metabolism. Affected individuals produce virtually no circulating apolipoprotein B-containing lipoproteins (chylomicrons, VLDL, LDL, lipoprotein(A)). Malabsorption of the antioxidant vitamin E occurs, leading to spinocerebellar and retinal degeneration.

Protein type: Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: endoplasmic reticulum lumen; receptor complex

Molecular Function: lipid transporter activity; lipid binding

Biological Process: lipoprotein metabolic process; lipid metabolic process; lipid transport

Disease: Abdominal Obesity-metabolic Syndrome 1; Abetalipoproteinemia