Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Typical Testing Data/Standard Curve (for reference only)

Mouse MDC / CCL22 ELISA Kit | MDC / CCL22 elisa kit

MDC / CCL22 Mouse ELISA Kit

Reactivity
Mouse
Applications
ELISA
Synonyms
MDC / CCL22; MDC / CCL22 Mouse ELISA Kit; Mouse MDC / CCL22; MDC / CCL22 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Applicable Applications for MDC / CCL22 elisa kit
Quantitative sELISA (EIA)
Application Notes
For quantitative detection of MDC in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Assay Type
Sandwich
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
< 1pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MDC / CCL22 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MDC polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MDC polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MDC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MDC can be calculated.

Background: Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22), is a protein that in humans is encoded by the CCL22 gene. The gene is located in human chromosome 16 in a cluster with other chemokines called CX3CL1 and CCL17. It is secreted by dendritic cells and macrophages, and elicits its effects on its target cells by interacting with cell surface chemokine receptors such as CCR4. HTLV-1-induced CCL22 causes the high frequency of FOXP3-positive cells observed in HTLV-1 infection and that FOXP3-positive cells may both retard the progression of ATLL and HTLV-1-associated inflammatory diseases and contribute to the immune suppression seen in HTLV-1 infection.

Similar Products

Product Notes

The Mouse MDC / CCL22 (Catalog #AAA355437) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA355437 ELISA Kit recognizes Mouse MDC / CCL22. AAA Biotech's MDC / CCL22 can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA). For quantitative detection of MDC in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants. Researchers should empirically determine the suitability of the MDC / CCL22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MDC / CCL22, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.