Mouse CD40 / TNFRSF5 ELISA Kit | CD40 / TNFRSF5 elisa kit

CD40 / TNFRSF5 Mouse ELISA Kit

Cat. Num. AAA355327

• Reactivity: Mouse
• Applications: ELISA
• Synonyms: CD40 / TNFRSF5; CD40 / TNFRSF5 Mouse ELISA Kit; Mouse CD40 / TNFRSF5; CD40 / TNFRSF5 elisa kit

• Reactivity: Mouse

• Applicable Applications for CD40 / TNFRSF5 elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of CD40 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
• Assay Type: Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: < 1pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD40 / TNFRSF5 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CD40 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CD40 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CD40 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CD40 can be calculated.

Background: CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. It is expressed on the surface of all mature B cells. This protein receptor is a member of the TNF-receptor superfamily. It has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 114053977
• NCBI Official Full Name: CD40

NCBI Description

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]