Human Furin ELISA Kit | FURIN elisa kit

Human Furin ELISA Kit

Cat. Num. AAA3803589

• Gene Names: FURIN; FUR; PACE; SPC1; PCSK3
• Reactivity: Human
• Synonyms: Furin; Human Furin ELISA Kit; FURIN elisa kit

• Reactivity: Human
• Sequence Length: 794

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 12.5 IU/L-200 IU/L
• Sensitivity: 1 IU/L

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FURIN elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofFURINin the sample, This FURINELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusFURINconcentration. The concentration ofFURINin the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 577019578
• NCBI GeneID: 5045
• NCBI Accession #: NP_001276752.1
• NCBI GenBank Nucleotide #: NP_001276752.1
• UniProt Accession #: P09958
• NCBI Official Full Name: furin preproprotein
• NCBI Official Synonym Full Names: furin, paired basic amino acid cleaving enzyme
• NCBI Official Symbol: FURIN
• NCBI Official Synonym Symbols: FUR; PACE; SPC1; PCSK3
• NCBI Protein Information: furin
• UniProt Protein Name: Furin
• Protein Family: Furin
• UniProt Gene Name: FURIN
• UniProt Synonym Gene Names: FUR; PACE; PCSK3; PACE
• UniProt Entry Name: FURIN_HUMAN

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. The product of this gene is one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is also thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140 and may play a role in tumor progression. This gene is located in close proximity to family member proprotein convertase subtilisin/kexin type 6 and upstream of the FES oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

FURIN: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Belongs to the peptidase S8 family. Furin subfamily.

Protein type: Cell surface; EC 3.4.21.75; Membrane protein, integral; Protease; Vesicle

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: cell surface; endoplasmic reticulum; extracellular space; Golgi lumen; Golgi membrane; lipid raft; membrane; plasma membrane; trans-Golgi network; trans-Golgi network transport vesicle

Molecular Function: endopeptidase activity; nerve growth factor binding; peptidase activity; peptide binding; protease binding; protein binding; serine-type endopeptidase activity; serine-type endopeptidase inhibitor activity

Biological Process: cell proliferation; cellular protein metabolic process; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; negative regulation of low-density lipoprotein receptor catabolic process; negative regulation of nerve growth factor production; negative regulation of transforming growth factor-beta1 production; nerve growth factor processing; nerve growth factor production; peptide biosynthetic process; peptide hormone processing; positive regulation of membrane protein ectodomain proteolysis; protein processing; regulation of endopeptidase activity; regulation of protein catabolic process; secretion by cell; signal peptide processing; transforming growth factor beta receptor signaling pathway; viral infectious cycle; viral protein processing