Typical Testing Data/Standard Curve (for reference only)
Mouse MCP-1 / CCL2 ELISA Kit | MCP-1 / CCL2 elisa kit
MCP-1 / CCL2 Mouse ELISA Kit
Reactivity
Mouse
Applications
ELISA
Synonyms
MCP-1 / CCL2; MCP-1 / CCL2 Mouse ELISA Kit; Mouse MCP-1 / CCL2; MCP-1 / CCL2 elisa kit
Reactivity
Mouse
Applicable Applications for MCP-1 / CCL2 elisa kit
Quantitative sELISA (EIA)
Application Notes
For quantitative detection of MCP-1 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Samples
Mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants
Assay Type
Quantitative Sandwich
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
< 6 pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MCP-1 / CCL2 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MCP-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MCP-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MCP-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MCP-1 can be calculated.!!Background/Introduction: Monocyte chemotactic protein-1(MCP-1), known as chemmkine(C-C motif) ligand 2(CCL2) or small inducible cytokine A2, is a small cytokine belonging to the CC chemokine family. The CCL2 gene is located on chromosome 17 (17q11.2-q21.1) in humans, and spans 1,927 bases. It has three exons and two introns, and produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. The predicted weight is 11.025 kiloDaltons (kDa). MCP-1 has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It induces amylin expression through ERK1/ ERK2/ JNK-AP1 and NF-kappaB related signaling pathways independent of CCR2. Amylin upregulation by CCL2 may contribute to elevation of plasma amylin in obesity and insulin resistance.
Similar Products
Product Notes
The Mouse MCP-1 / CCL2 (Catalog #AAA355393) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA355393 ELISA Kit recognizes Mouse MCP-1 / CCL2. AAA Biotech's MCP-1 / CCL2 can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA). For quantitative detection of MCP-1 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants. Researchers should empirically determine the suitability of the MCP-1 / CCL2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCP-1 / CCL2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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