Rabbit anti-Human ARCN1 Polyclonal Antibody | anti-ARCN1 antibody

ARCN1, ID (ARCN1, COPD, Coatomer subunit delta, Archain, Delta-coat protein) (APC)

Cat. Num. AAA6274710

• Gene Names: ARCN1; COPD
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ARCN1; Polyclonal Antibody; ID (ARCN1; COPD; Coatomer subunit delta; Archain; Delta-coat protein) (APC); Delta-coat protein; anti-ARCN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ARCN1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ARCN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-324 amino acids from the Central region of human ARCN1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ARCN1 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ARCN1 antibody detected the ARCN1 protein (arrow).)

Related Product Information for anti-ARCN1 antibody

This gene maps in a region, which include the mixed lineage leukemia and Friend leukemia virus integration 1 genes, where multiple disease-associated chromosome translocations occur. It is an intracellular protein. Archain sequences are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking.

Product Categories/Family for anti-ARCN1 antibody

Antibodies; Proteins

References

Lippincott-Schwartz, J., et al. Trends Cell Biol. 16 (10), E1-E4 (2006): Xu, Y., et al. Mol. Biol. Cell 13(10):3493-3507(2002). Lippincott-Schwartz, J., et al. Annu. Rev. Cell Dev. Biol. 16, 557-589 (2000): Lowe, M., et al. J. Biol. Chem. 271(48):30725-30730(1996). Tunnacliffe, A., et al. Mamm. Genome 7(10):784-786(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 214010212
• NCBI GeneID: 372
• NCBI Accession #: NP_001135753.1
• NCBI GenBank Nucleotide #: NM_001142281.1
• UniProt Accession #: P48444; Q52M80; B4E1X2; E9PEU4
• Molecular Weight: 57,210 Da
• NCBI Official Full Name: coatomer subunit delta isoform 2
• NCBI Official Synonym Full Names: archain 1
• NCBI Official Symbol: ARCN1
• NCBI Official Synonym Symbols: COPD
• NCBI Protein Information: coatomer subunit delta; delta-COP; delta-coat protein; coatomer delta subunit; coatomer protein delta-COP; archain vesicle transport protein 1; coatomer protein complex, subunit delta
• UniProt Protein Name: Coatomer subunit delta
• Protein Family: Coatomer
• UniProt Gene Name: ARCN1
• UniProt Synonym Gene Names: COPD; Delta-COP
• UniProt Entry Name: COPD_HUMAN

Uniprot Description

ARCN1: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: Golgi apparatus; clathrin adaptor complex; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; COPI vesicle coat; cytosol

Biological Process: cerebellar Purkinje cell layer maturation; intracellular protein transport; COPI coating of Golgi vesicle; pigmentation; adult locomotory behavior; retrograde vesicle-mediated transport, Golgi to ER