Typical Testing Data/Standard Curve (for reference only)
Mouse Major urinary protein 1 (MUP1) ELISA Kit | MUP1 elisa kit
Major urinary protein 1 (MUP1): Mouse (ELISA)
Reactivity
Mouse
Synonyms
Major urinary protein 1 (MUP1); Major urinary protein 1 (MUP1): Mouse (ELISA); Mouse major urinary protein 1 (MUP1); MUP1 elisa kit
Reactivity
Mouse
Specificity
The antibodies used in this assay are specific to mouse Mup1.
Samples
Serum and plasma
Assay Type
Quantitative Sandwich
Sensitivity
0.625 ng/ml
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MUP1 elisa kit
Intended Uses: This Mup1 ELISA kit is designed for quantification of mouse Mup1 in serum and plasma samples.
Principle of the Assay: This assay is a sandwich ELISA designed for the quantitative detection of mouse Mup1 in samples. The immunoplate is pre-coated with antibody specific to mouse Mup1. Standards and samples are pipetted into the wells and any mouse Mup1 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for mouse Mup1 is added to the wells. After a final wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse Mup1 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse Mup1, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Background/Introduction: Major urinary protein 1 (Mup1), also known as Mup7, Up-1, Ltn-1, Mup-1, Mup-a, Mup10 and Lvtn-1, is a low molecular weight secreted protein produced predominantly from the liver. [1] Structurally it belongs to the lipocalin family, which carries small hydrophobic ligands such as pheromones. It has been demonstrated that Mup1 is an important player in regulating energy expenditure and metabolism in mice. In both dietary and genetic obese mice, the circulating concentrations of Mup1 were markedly decreased compared with the lean controls. Replenishment of recombinant MUP-1 led to improved glucose tolerance and insulin sensitivity, as well as increased energy expenditure and locomotor activity in db/db diabetic mice. [2] It was suggested that Mup1 also regulates systemic glucose and/or lipid metabolism through the paracrine/autocrine regulation of the hepatic gluconeogenic and/or lipogenic programs, respectively. [3]
Principle of the Assay: This assay is a sandwich ELISA designed for the quantitative detection of mouse Mup1 in samples. The immunoplate is pre-coated with antibody specific to mouse Mup1. Standards and samples are pipetted into the wells and any mouse Mup1 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for mouse Mup1 is added to the wells. After a final wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse Mup1 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse Mup1, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Background/Introduction: Major urinary protein 1 (Mup1), also known as Mup7, Up-1, Ltn-1, Mup-1, Mup-a, Mup10 and Lvtn-1, is a low molecular weight secreted protein produced predominantly from the liver. [1] Structurally it belongs to the lipocalin family, which carries small hydrophobic ligands such as pheromones. It has been demonstrated that Mup1 is an important player in regulating energy expenditure and metabolism in mice. In both dietary and genetic obese mice, the circulating concentrations of Mup1 were markedly decreased compared with the lean controls. Replenishment of recombinant MUP-1 led to improved glucose tolerance and insulin sensitivity, as well as increased energy expenditure and locomotor activity in db/db diabetic mice. [2] It was suggested that Mup1 also regulates systemic glucose and/or lipid metabolism through the paracrine/autocrine regulation of the hepatic gluconeogenic and/or lipogenic programs, respectively. [3]
References
1. Major urinary protein-1 increases energy expenditure and improves glucose intolerance through enhancing mitochondrial function in skeletal muscle of diabetic mice. Hui X, et al J Biol Chem. 2009 May 22;284(21):14050-7.
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Product Notes
The Mouse MUP1 (Catalog #AAA398063) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA398063 ELISA Kit recognizes Mouse MUP1. It is sometimes possible for the material contained within the vial of "Major urinary protein 1 (MUP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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