Human Lipocalin-2 protein (NGAL) ELISA Kit | LCN2 elisa kit

Lipocalin-2 protein (NGAL): Human (ELISA)

Cat. Num. AAA398048

• Gene Names: LCN2; p25; 24p3; MSFI; NGAL
• Reactivity: Human
• Synonyms: Lipocalin-2 protein (NGAL); Lipocalin-2 protein (NGAL): Human (ELISA); Human lipocalin 2 (NGAL); LCN2 elisa kit

• Reactivity: Human
• Specificity: The antibody pair used in this assay is specific to human lipocalin-2 and does not cross-react with mouse and rat lipocalin-2, and other cytokine or hormone molecules.
• Sequence Length: 198

• Samples: Serum, plasma, urine and cell culture supernate samples
• Assay Type: Quantitative Sandwich
• Sensitivity: 0.39 ng/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LCN2 elisa kit

Intended Uses: This Human lipocalin-2 ELISA kit is designed for quantification of human lipocalin-2 in serum, plasma, urine and cell culture supernate samples.

Principle of the Assay: This assay is a quantitative sandwich ELISA using monoclonal antibodies against human LCN2. The immunoplate is pre-coated with a monoclonal antibody specific for human LCN2. Standards and samples are added into the wells and any human LCN2 present is captured by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked monoclonal antibody specific for human LCN2 is added to the wells. After a final washing step to remove any unbound reagents, a HRP substrate solution is added and colour develops in proportion to the amount of human LCN2 bound initially. The reaction is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human LCN2, the unknown sample concentration can be interpolated from a reference curve included in each assay.

Background/Introduction: Lipocalin-2(LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), 24p3, or neutrophil lipocalin (NL), is a 25-kDa secretory glycoprotein1. LCN2 has been implicated in a variety of cellular processes including the innate immune response, differentiation, tumorigenesis, and cell survival2,3. It appears to be upregulated in various inflammation and infection conditions. Several reports suggest that LCN2 may represent a sensitive biomarker for various renal injuries4 and is associated with several types of cancers, including breast cancer5, ovarian, colorectal, and pancreatic cancers6,7. Furthermore, a growing body of evidence suggests that serum levels of lipocalin-2 are correlated with obesity, insulin resistance, hyperglycemia, coronary heart disease and fatty liver disease in humans8-11.

References

1. A emerging clinical and experimental evidence for the role of lipocalin-2 in metabolic syndrome. Jang Y, Lee JH, Wang Y, Sweeney G. Clin Exp Pharmacol Physiol. 2012 Feb;39(2):194-9
2. The multifaceted roles of neutrophil gelatinase associated lipocalin (NGAL) in inflammation and cancer. Chakraborty S, Kaur S, Guha S, Batra SK. Biochim Biophys Acta. 2012 Aug;1826(1):129-69.

NCBI and Uniprot Product Information

• NCBI GI #: 21619839
• NCBI GeneID: 3934
• NCBI Official Full Name: Lipocalin 2
• NCBI Official Synonym Full Names: lipocalin 2
• NCBI Official Symbol: LCN2
• NCBI Official Synonym Symbols: p25; 24p3; MSFI; NGAL
• NCBI Protein Information: neutrophil gelatinase-associated lipocalin
• Protein Family: Neutrophil gelatinase-associated lipocalin

NCBI Description

This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015]