Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Lipoprotein-associated phospholipase A2 (Lp-PLA2) Standard Curve.)
Mouse Lipoprotein-Associated Phospholipase A2 (Lp-PLA2) ELISA Kit | Lp-PLA2 elisa kit
Mouse Lipoprotein-Associated Phospholipase A2 (Lp-PLA2) ELISA Kit
Gene Names
Pla2g7; PAF-AH; R75400
Reactivity
Mouse
Synonyms
Lipoprotein-Associated Phospholipase A2 (Lp-PLA2); Mouse Lipoprotein-Associated Phospholipase A2 (Lp-PLA2) ELISA Kit; LP-PLA2; LDL-PLA2; PLA2G7; PAFAH; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; PAF 2-acylhydrolase; lipoprotein-associated phospholipase A2; phospholipase A2; Lp-PLA2 elisa kit
Reactivity
Mouse
Specificity
Mouse Lipoprotein-associated phospholipase A2 (Lp-PLA2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LP-PLA2. No significant cross-reactivity or interference between Mouse LP-PLA2 and analogues was observed.
Sequence Length
440
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Lipoprotein-associated phospholipase A2 (Lp-PLA2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Lipoprotein-associated phospholipase A2 (Lp-PLA2) Standard Curve.)
Related Product Information for Lp-PLA2 elisa kit
Description: This Mouse Lipoprotein-associated phospholipase A2 (Lp-PLA2) ELISA Kit employs a two-site sandwich ELISA to quantitate LP-PLA2 in samples. An antibody specific for LP-PLA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LP-PLA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LP-PLA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LP-PLA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Lipoprotein-associated phospholipase A2 (Lp-PLA2, also known as platelet activating factor acetyl-hydrolase), is a relatively recent marker that has emerged as a major, independent predictor of CHD. Lp-PLA2 is an active phospholipase that circulates in plasma bound to LDL, and it appears to act almost exclusively on oxidized LDL to generate lysophosphatidyl-choline, which is proinflammatory and is considered to be atherogenic. Thus, Lp-PLA2 represents an interesting link between lipoproteinoxidation and vascular inflammation, which likely explain its strong, independent association with CHD risk. Data from the recent ARIC study have indicated that the predictive power of Lp-PLA2 is attenuated after adjustment for multiple established risk factors.
Background: Lipoprotein-associated phospholipase A2 (Lp-PLA2, also known as platelet activating factor acetyl-hydrolase), is a relatively recent marker that has emerged as a major, independent predictor of CHD. Lp-PLA2 is an active phospholipase that circulates in plasma bound to LDL, and it appears to act almost exclusively on oxidized LDL to generate lysophosphatidyl-choline, which is proinflammatory and is considered to be atherogenic. Thus, Lp-PLA2 represents an interesting link between lipoproteinoxidation and vascular inflammation, which likely explain its strong, independent association with CHD risk. Data from the recent ARIC study have indicated that the predictive power of Lp-PLA2 is attenuated after adjustment for multiple established risk factors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
platelet-activating factor acetylhydrolase
NCBI Official Synonym Full Names
phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
NCBI Official Symbol
Pla2g7
NCBI Official Synonym Symbols
PAF-AH; R75400
NCBI Protein Information
platelet-activating factor acetylhydrolase
UniProt Protein Name
Platelet-activating factor acetylhydrolase
UniProt Gene Name
Pla2g7
UniProt Synonym Gene Names
Pafah; PAF acetylhydrolase; LDL-PLA(2)
Uniprot Description
Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.
Research Articles on Lp-PLA2
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Product Notes
The Mouse Lp-PLA2 pla2g7 (Catalog #AAA9717345) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717345 ELISA Kit recognizes Mouse Lp-PLA2. It is sometimes possible for the material contained within the vial of "Lipoprotein-Associated Phospholipase A2 (Lp-PLA2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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