Typical Testing Data/Standard Curve (for reference only)
Human phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) ELISA Kit | PLA2G7 elisa kit
Human lipoprotein-associated phospholipase A2, Lp-PLA2 ELISA Kit
Gene Names
PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2
Reactivity
Human
Synonyms
phospholipase A2; group VII (platelet-activating factor acetylhydrolase; plasma); Human lipoprotein-associated phospholipase A2; Lp-PLA2 ELISA Kit; LDL-PLA2; LP-PLA2; PAFAH; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; PAF 2-acylhydrolase; lipoprotein-associated phospholipase A2; ; PLA2G7 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human Lp-PLA2. No significant cross-reactivity or interference between human Lp-PLA2 and analogues was observed.
Sequence Length
441
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
1.56 ng/ml-100 ng/ml
Sensitivity
< 0.39 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLA2G7 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Lp-PLA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Lp-PLA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Lp-PLA2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Lp-PLA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,077 Da
NCBI Official Full Name
platelet-activating factor acetylhydrolase
NCBI Official Synonym Full Names
phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
NCBI Official Symbol
PLA2G7
NCBI Official Synonym Symbols
PAFAD; PAFAH; LP-PLA2; LDL-PLA2
NCBI Protein Information
platelet-activating factor acetylhydrolase; LDL-PLA(2); gVIIA-PLA2; PAF 2-acylhydrolase; PAF acetylhydrolase; group-VIIA phospholipase A2; LDL-associated phospholipase A2; lipoprotein-associated phospholipase A2; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase
UniProt Protein Name
Platelet-activating factor acetylhydrolase
Protein Family
UniProt Gene Name
PLA2G7
UniProt Synonym Gene Names
PAFAH; PAF acetylhydrolase; gVIIA-PLA2; LDL-PLA(2)
UniProt Entry Name
PAFA_HUMAN
NCBI Description
The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2009]
Uniprot Description
Function: Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.
Catalytic activity: 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.
Research Articles on PLA2G7
Similar Products
Product Notes
The Human PLA2G7 pla2g7 (Catalog #AAA703495) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA703495 ELISA Kit recognizes Human PLA2G7. It is sometimes possible for the material contained within the vial of "phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
