PLA2G7 / PAFAH recombinant protein

Mouse PLA2G7 / PAFAH Protein (His Tag)

Cat. Num. AAA2546593

• Gene Names: Pla2g7; PAF-AH; R75400
• Purity: > 90 % as determined by SDS-PAGE.
• Synonyms: PLA2G7 / PAFAH; Mouse PLA2G7 / PAFAH Protein (His Tag); Pafah; PLA2G7 / PAFAH recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 440

• Application Notes: The recombinant mouse Pla2g7 consists of 430 amino acids and predicts a molecular mass of 48.3 kDa.
• Predicted N Terminal: Phe 22
• Endotoxin: < 1.0 EU per mug protein as determined by the LAL method.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PLA2G7 / PAFAH recombinant protein

Background: Platelet-activating factor acetylhydrolase, also known as 1-alkyl-2-acetylglycerophosphocholine esterase, 2-acetyl-1-alkylglycero-phosphocholine esterase, Group-VIIA phospholipase A2, LDL-associated phospholipase A2, PAF 2-acylhydrolase, PLA2G7 and PAFAH, is secreted protein which belongs to the AB hydrolase superfamily and Lipase family. PLA2G7 / PAFAH modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. It has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. PLA2G7 / PAFAH is a potent pro- and anti-inflammatory molecule that has been implicated in multiple inflammatory disease processes, including cardiovascular disease. PLA2G7 also represents an important, potentially functional candidate in the pathophysiology of coronary artery disease (CAD). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PLA2G7 deficiency). It is a trait which is present in 27% of Japanese. It could have a significant physiologic effect in the presence of inflammatory bodily responses.

Description: A DNA sequence encoding the mouse Pla2g7 (NP_038765.2) (Met1-Asn440) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 31980752
• NCBI GeneID: 27226
• NCBI Accession #: NP_038765.2
• NCBI GenBank Nucleotide #: NM_013737.5
• UniProt Accession #: Q60963; Q8BKM3; Q921T4
• Molecular Weight: 49,258 Da
• NCBI Official Full Name: platelet-activating factor acetylhydrolase
• NCBI Official Synonym Full Names: phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
• NCBI Official Symbol: Pla2g7
• NCBI Official Synonym Symbols: PAF-AH; R75400
• NCBI Protein Information: platelet-activating factor acetylhydrolase
• UniProt Protein Name: Platelet-activating factor acetylhydrolase
• UniProt Gene Name: Pla2g7
• UniProt Synonym Gene Names: Pafah; PAF acetylhydrolase; LDL-PLA(2)

Uniprot Description

PLA2G7: Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PAFAD). An enzymatic deficiency that results in exacerbated bodily response to inflammatory agents. Asthmatic individuals affected by this condition may manifest severe respiratory symptoms. Defects in PLA2G7 are a cause of susceptibility to asthma (ASTHMA). The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi. PLA2G7 variants can be a risk factor for the development of asthma and PLA2G7 may act as a modifier gene that modulates the severity of this disease. Defects in PLA2G7 are a cause of susceptibility to atopic hypersensitivity (ATOPY). A condition characterized by predisposition to develop hypersensitivity reactions. Atopic individuals can develop eczema, allergic rhinitis and allergic asthma. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: EC 3.1.1.47; Hydrolase; Lipid Metabolism - ether lipid; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17|17 B3

Cellular Component: cytoplasm; extracellular space

Molecular Function: 1-alkyl-2-acetylglycerophosphocholine esterase activity; calcium-independent phospholipase A2 activity; phospholipid binding

Biological Process: inflammatory response; platelet activating factor metabolic process