Mouse Lipase, Hepatic (LIPC) ELISA Kit | LIPC elisa kit

Mouse Lipase, Hepatic (LIPC) ELISA Kit

Cat. Num. AAA2021723

• Gene Names: LIPC; HL; HTGL; LIPH; HDLCQ12
• Reactivity: Mouse
• Synonyms: Lipase; Hepatic (LIPC); Mouse Lipase; Hepatic (LIPC) ELISA Kit; HL; HTGL; Hepatic Lipase; Hepatic triacylglycerol lipase; Lipase member C; LIPC elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lipase, Hepatic (LIPC).; No significant cross-reactivity or interference between Lipase, Hepatic (LIPC) and analogues was observed.
• Sequence Length: 499

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.126ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase, Hepatic (LIPC) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase, Hepatic (LIPC) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LIPC elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Hepatic (LIPC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Hepatic (LIPC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Hepatic (LIPC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase, Hepatic (LIPC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LIPC elisa kit

Enzyme & Kinase; Metabolic pathway; Cardiovascular biology

NCBI and Uniprot Product Information

• NCBI GI #: 119597942
• NCBI GeneID: 3990
• UniProt Accession #: P11150; O43571; P78529; Q99465; A2RUB4; A8K9B6
• Molecular Weight: 55,914 Da
• NCBI Official Full Name: lipase, hepatic
• NCBI Official Synonym Full Names: lipase, hepatic
• NCBI Official Symbol: LIPC
• NCBI Official Synonym Symbols: HL; HTGL; LIPH; HDLCQ12
• NCBI Protein Information: hepatic triacylglycerol lipase
• UniProt Protein Name: Hepatic triacylglycerol lipase
• Protein Family: Spore germination lipase
• UniProt Gene Name: LIPC
• UniProt Synonym Gene Names: HTGL; HL; Hepatic lipase
• UniProt Entry Name: LIPC_HUMAN

NCBI Description

LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. [provided by RefSeq, Jul 2008]

Uniprot Description

LIPC: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin. Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency). A disorder characterized by elevated levels of beta-migrating very low density lipoproteins, and abnormally triglyceride-rich low and high density lipoproteins. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Secreted, signal peptide; Secreted; Lipid Metabolism - glycerolipid; Phospholipase; EC 3.1.1.3

Chromosomal Location of Human Ortholog: 15q21-q23

Cellular Component: extracellular space

Molecular Function: heparin binding; triacylglycerol lipase activity; low-density lipoprotein binding; apolipoprotein binding; phospholipase activity

Biological Process: cholesterol metabolic process; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol catabolic process; fatty acid biosynthetic process

Disease: High Density Lipoprotein Cholesterol Level Quantitative Trait Locus 12; Hepatic Lipase Deficiency; Diabetes Mellitus, Noninsulin-dependent