Goat Hepatic lipase ELISA Kit | HL elisa kit

Goat Hepatic lipase ELISA Kit

Cat. Num. AAA741238

• Gene Names: LIPC; HL; HTGL; LIPH; HDLCQ12
• Reactivity: Goat
• Synonyms: Hepatic lipase; Goat Hepatic lipase ELISA Kit; HL elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HL elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This LIPC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat LIPC. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY LIPC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LIPC antibody and an LIPC-HRP conjugate. The assay sample and buffer are incubated together with LIPC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LIPC concentration since LIPC from samples and LIPC-HRP conjugate compete for the anti-LIPC antibody binding site. Since the number of sites is limited, as more sites are occupied by LIPC from the sample, fewer sites are left to bind LIPC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LIPC concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 194097335
• NCBI GeneID: 3990
• NCBI Accession #: NP_000227.2
• NCBI GenBank Nucleotide #: NM_000236.2
• Molecular Weight: 55,914 Da
• NCBI Official Full Name: hepatic triacylglycerol lipase
• NCBI Official Synonym Full Names: lipase, hepatic
• NCBI Official Symbol: LIPC
• NCBI Official Synonym Symbols: HL; HTGL; LIPH; HDLCQ12
• NCBI Protein Information: hepatic triacylglycerol lipase; hepatic lipase; lipase member C
• UniProt Protein Name: Hepatic triacylglycerol lipase
• UniProt Gene Name: LIPC
• UniProt Synonym Gene Names: HTGL; HL; Hepatic lipase
• UniProt Entry Name: LIPC_HUMAN

NCBI Description

LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. [provided by RefSeq, Jul 2008]

Uniprot Description

LIPC: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin. Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency). A disorder characterized by elevated levels of beta-migrating very low density lipoproteins, and abnormally triglyceride-rich low and high density lipoproteins. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Secreted, signal peptide; Phospholipase; Lipid Metabolism - glycerolipid; Secreted; EC 3.1.1.3

Chromosomal Location of Human Ortholog: 15q21-q23

Cellular Component: extracellular space

Molecular Function: heparin binding; triacylglycerol lipase activity; low-density lipoprotein binding; apolipoprotein binding; phospholipase activity

Biological Process: cholesterol metabolic process; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol catabolic process; fatty acid biosynthetic process

Disease: High Density Lipoprotein Cholesterol Level Quantitative Trait Locus 12; Hepatic Lipase Deficiency; Diabetes Mellitus, Noninsulin-dependent