Mouse Lipase, Hepatic ELISA Kit | LIPC elisa kit

Mouse Lipase, Hepatic ELISA Kit

Cat. Num. AAA761501

• Gene Names: LIPC; HL; HTGL; LIPH; HDLCQ12
• Reactivity: Mouse
• Synonyms: Lipase; Hepatic; Mouse Lipase; Hepatic ELISA Kit; LIPC/HTGL/Hepatic triacylglycerol lipase/HL/Hepatic lipase/Lipase member C; LIPC elisa kit

• Reactivity: Mouse
• Sequence Length: 499

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.391-25ng/ml
• Sensitivity: 0.234ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for LIPC elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse LIPC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse LIPC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse LIPC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse LIPC can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119597942
• NCBI GeneID: 3990
• Molecular Weight: 55,914 Da
• NCBI Official Full Name: lipase, hepatic
• NCBI Official Synonym Full Names: lipase C, hepatic type
• NCBI Official Symbol: LIPC
• NCBI Official Synonym Symbols: HL; HTGL; LIPH; HDLCQ12
• NCBI Protein Information: hepatic triacylglycerol lipase
• UniProt Protein Name: Hepatic triacylglycerol lipase
• Protein Family: Spore germination lipase
• UniProt Gene Name: LIPC
• UniProt Synonym Gene Names: HTGL; HL; Hepatic lipase
• UniProt Entry Name: LIPC_HUMAN

NCBI Description

LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. [provided by RefSeq, Jul 2008]

Uniprot Description

LIPC: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin. Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency). A disorder characterized by elevated levels of beta-migrating very low density lipoproteins, and abnormally triglyceride-rich low and high density lipoproteins. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Lipid Metabolism - glycerolipid; Secreted; EC 3.1.1.3; Secreted, signal peptide; Phospholipase

Chromosomal Location of Human Ortholog: 15q21-q23

Cellular Component: cell surface; early endosome; endoplasmic reticulum lumen; extracellular region; extracellular space; late endosome; microvillus

Molecular Function: acylglycerol lipase activity; apolipoprotein binding; heparan sulfate proteoglycan binding; heparin binding; lipid binding; low-density lipoprotein binding; lysophospholipase activity; phospholipase A1 activity; phospholipase activity; transferase activity, transferring acyl groups; triacylglycerol lipase activity

Biological Process: cellular response to hormone stimulus; cholesterol homeostasis; cholesterol metabolic process; circadian rhythm; developmental growth; fatty acid biosynthetic process; heparan sulfate proteoglycan biosynthetic process; lipid digestion; liver development; phosphatidic acid metabolic process; phosphatidylethanolamine metabolic process; phosphatidylserine metabolic process; protein oligomerization; response to acetate; response to amino acid stimulus; response to calcium ion; response to carbohydrate stimulus; response to copper ion; response to drug; response to glucocorticoid stimulus; response to hypoxia; response to magnesium ion; response to nutrient levels; response to peptide hormone stimulus; reverse cholesterol transport; triacylglycerol catabolic process

Disease: Diabetes Mellitus, Noninsulin-dependent; Hepatic Lipase Deficiency; High Density Lipoprotein Cholesterol Level Quantitative Trait Locus 12