Testing Data
Rabbit anti-Mouse IL-1 beta Polyclonal Antibody | anti-IL-1 beta antibody
Anti-Mouse IL-1 beta Antibody
Gene Names
IL1B; IL-1; IL1F2; IL1-BETA
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA
Purity
Purified
Synonyms
IL-1 beta; Polyclonal Antibody; Anti-Mouse IL-1 beta Antibody; Mouse IL-1 beta; anti-IL-1 beta antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
This antiserum has been heated to 56 degree C for 30 minutes. This antibody is primarily directed against mature, 17,000 MW mouse IL-1b and is useful in determining its presence in various assays. The antiserum does not recognize human IL-1b or mouse IL-1a. In ELISA formats and other immunoreactive assays, reactivity with rat IL-1b is variable and depends on the capture antibody. This antiserum will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1b containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL-1b precursor molecule. Denatured 31,000 precursor IL-1b will be recognized by this antibody. This is due to the unfolding of the denatured precursor IL-1b exposing epitopes not exposed in the natural state. This antibody is also useful for neutralization of mouse IL-1b activity in bioassays. Rat IL-1b activity is neutralized incompletely. It does not neutralize the biological activity of mouse IL-1a. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Purity/Purification
Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
269
Applicable Applications for anti-IL-1 beta antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (EIA)
Application Notes
This antiserum against Mouse IL-1b has been tested for use in neutralizations, ELISA, radioimmunoassays, immunoblotting, immunohistochemistry and immunoprecipitation. It recognizes the 17,000 MW mature Mouse IL-1b. Reactivity in other immunoassays is unknown.
Immunogen
The whole rabbit serum was prepared by repeated immunizations with recombinant mouse IL-1b produced in E.coli. The MW of the recombinant IL-1b was 17 kDa.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interleukin-1 beta proprotein
NCBI Official Synonym Full Names
interleukin 1 beta
NCBI Official Symbol
IL1B
NCBI Official Synonym Symbols
IL-1; IL1F2; IL1-BETA
NCBI Protein Information
interleukin-1 beta
UniProt Protein Name
Interleukin-1 beta
UniProt Gene Name
IL1B
UniProt Synonym Gene Names
IL1F2; IL-1 beta
UniProt Entry Name
IL1B_HUMAN
NCBI Description
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Research Articles on IL-1 beta
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Product Notes
The IL-1 beta il1b (Catalog #AAA4156822) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse IL-1 beta Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL-1 beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (EIA). This antiserum against Mouse IL-1b has been tested for use in neutralizations, ELISA, radioimmunoassays, immunoblotting, immunohistochemistry and immunoprecipitation. It recognizes the 17,000 MW mature Mouse IL-1b. Reactivity in other immunoassays is unknown. Researchers should empirically determine the suitability of the IL-1 beta il1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-1 beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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