Mouse Interleukin 23 Subunit Alpha (IL23a) ELISA Kit | IL23a elisa kit

Mouse Interleukin 23 Subunit Alpha (IL23a) Mini Samples ELISA Kit

Cat. Num. AAA2708291

• Gene Names: Il23a; p19; IL-23
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Interleukin 23 Subunit Alpha (IL23a); Mouse Interleukin 23 Subunit Alpha (IL23a) Mini Samples ELISA Kit; Interleukin 23 Subunit Alpha; P19; IL23P19; SGRF; IL23a elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 23 Subunit Alpha (IL23a).; No significant cross-reactivity or interference between Mini Samples Interleukin 23 Subunit Alpha (IL23a) and analogues was observed.
• Sequence Length: 196

• Applicable Applications for IL23a elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 7.8-500pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 3.2pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 23 Subunit Alpha (IL23a) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 23 Subunit Alpha (IL23a) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 23 Subunit Alpha (IL23a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 23 Subunit Alpha (IL23a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 23 Subunit Alpha (IL23a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Interleukin 23 Subunit Alpha (IL23a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 81881842
• NCBI GeneID: 83430
• UniProt Accession #: Q9EQ14
• NCBI Official Full Name: Interleukin-23 subunit alpha
• NCBI Official Synonym Full Names: interleukin 23, alpha subunit p19
• NCBI Official Symbol: Il23a
• NCBI Official Synonym Symbols: p19; IL-23
• NCBI Protein Information: interleukin-23 subunit alpha
• UniProt Protein Name: Interleukin-23 subunit alpha
• Protein Family: Interleukin
• UniProt Gene Name: Il23a
• UniProt Synonym Gene Names: IL-23 subunit alpha; IL-23-A; IL-23p19
• UniProt Entry Name: IL23A_MOUSE

Uniprot Description

IL23A: Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak- Stat signaling cascade, stimulates memory rather than naive T- cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Belongs to the IL-6 superfamily.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; cytokine activity; interleukin-23 receptor binding

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of T-helper 1 type immune response; negative regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of T cell mediated cytotoxicity; positive regulation of osteoclast differentiation; tissue remodeling; positive regulation of NK T cell proliferation; positive regulation of tyrosine phosphorylation of Stat4 protein; defense response to Gram-negative bacterium; positive regulation of activated T cell proliferation; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; T cell proliferation; positive regulation of T cell proliferation; inflammatory response; defense response to virus; positive regulation of memory T cell differentiation; positive regulation of interleukin-17 production; positive regulation of natural killer cell proliferation; positive regulation of NK T cell activation; immune system process; positive regulation of tumor necrosis factor production; regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of interferon-gamma production; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of defense response to virus by host