Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Interleukin 16 (IL-16) Standard Curve.)
Mouse Interleukin 16 (IL-16) ELISA Kit | IL-16 elisa kit
Mouse Interleukin 16 (IL-16) ELISA Kit
Gene Names
Il16; mKIAA4048
Reactivity
Mouse
Synonyms
Interleukin 16 (IL-16); Mouse Interleukin 16 (IL-16) ELISA Kit; IL16; FLJ16806; FLJ42735; FLJ44234; HsT19289; IL-16; LCF; prIL-16; OTTHUMP00000190849; OTTHUMP00000190850; interleukin 16; lymphocyte chemoattractant factor; IL-16 elisa kit
Reactivity
Mouse
Specificity
Mouse Interleukin 16 (IL-16) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IL16. No significant cross-reactivity or interference between Mouse IL16 and analogues was observed.
Sequence Length
1322
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Interleukin 16 (IL-16) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Interleukin 16 (IL-16) Standard Curve.)
Related Product Information for IL-16 elisa kit
Description: This Mouse Interleukin 16 (IL-16) ELISA Kit employs a two-site sandwich ELISA to quantitate IL16 in samples. An antibody specific for IL16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL16 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: IL-16 is constitutively expressed by both CD4+ and CD8+ T cells. Airway epithelium from asthmatics, eosinophils, and mast cells also produce IL-16, albeit only after stimulation.In lymphocytes, IL-16 is generated as a precursor molecule which is enzymatically cleaved following stimulation with T-cell mitogens. IL-16 is a proinflammatory and immunomodulatory cytokine.It is a potent chemoattractant for all CD4+ cells including T cells, monocytes, and eosinophils and, as such, has been identified at inflammatory sites characterized by infiltrating CD4+ cells.In addition to cell motility, IL-16 induces cell cycle progression and cytokine synthesis in CD4+ T cells.
Background: IL-16 is constitutively expressed by both CD4+ and CD8+ T cells. Airway epithelium from asthmatics, eosinophils, and mast cells also produce IL-16, albeit only after stimulation.In lymphocytes, IL-16 is generated as a precursor molecule which is enzymatically cleaved following stimulation with T-cell mitogens. IL-16 is a proinflammatory and immunomodulatory cytokine.It is a potent chemoattractant for all CD4+ cells including T cells, monocytes, and eosinophils and, as such, has been identified at inflammatory sites characterized by infiltrating CD4+ cells.In addition to cell motility, IL-16 induces cell cycle progression and cytokine synthesis in CD4+ T cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pro-interleukin-16 isoform 2
NCBI Official Synonym Full Names
interleukin 16
NCBI Official Symbol
Il16
NCBI Official Synonym Symbols
mKIAA4048
NCBI Protein Information
pro-interleukin-16
UniProt Protein Name
Pro-interleukin-16
UniProt Gene Name
Il16
UniProt Synonym Gene Names
IL-16; LCF
UniProt Entry Name
IL16_MOUSE
Uniprot Description
IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter.
Protein type: Cytokine; DNA-binding; Motility/polarity/chemotaxis
Cellular Component: extracellular space; cytoplasm; plasma membrane; extracellular region; intracellular; nucleus
Molecular Function: protein binding; cytokine activity
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; leukocyte chemotaxis; chemotaxis; induction of positive chemotaxis
Research Articles on IL-16
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Product Notes
The Mouse IL-16 il16 (Catalog #AAA9718148) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718148 ELISA Kit recognizes Mouse IL-16. It is sometimes possible for the material contained within the vial of "Interleukin 16 (IL-16), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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