Mouse Interleukin 10 (IL10) ELISA Kit | IL10 elisa kit

Mouse Interleukin 10 (IL10) Mini Samples ELISA Kit

Cat. Num. AAA2089483

• Gene Names: Il10; CSIF; Il-10
• Reactivity: Mouse
• Synonyms: Interleukin 10 (IL10); Mouse Interleukin 10 (IL10) Mini Samples ELISA Kit; CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor; IL10 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL10. No significant cross-reactivity or interference between IL10 and analogues was observed.
• Sequence Length: 178

• Samples: Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 18.8-1,200pg/mL
• Sensitivity: <7.1pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL10 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL10 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL10 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL10 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to IL10. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IL10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL10 elisa kit

Cytokine; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 6754318
• NCBI GeneID: 16153
• NCBI Accession #: NP_034678.1
• NCBI GenBank Nucleotide #: NM_010548.2
• UniProt Accession #: P18893; Q0VBJ1
• Molecular Weight: 20,641 Da
• NCBI Official Full Name: interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: Il10
• NCBI Official Synonym Symbols: CSIF; Il-10
• NCBI Protein Information: interleukin-10
• UniProt Protein Name: Interleukin-10
• Protein Family: Interleukin
• UniProt Gene Name: Il10
• UniProt Synonym Gene Names: Il-10; IL-10; CSIF

NCBI Description

This gene encodes an anti-inflammatory cytokine that is a member of the class-2 cytokine family. The encoded protein is secreted by cells of both the innate and adaptive immune systems and is crucial for limiting the immune response to a broad range of pathogens. It also has been shown to suppress autoimmune responses. This protein mediates it's immunosuppressive signal through a specific interleukin 10 receptor complex. Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, Sep 2015]

Uniprot Description

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.

Protein type: Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1 E4|1 56.89 cM

Cellular Component: extracellular space

Molecular Function: cytokine activity; interleukin-10 receptor binding

Biological Process: defense response to bacterium; defense response to protozoan; immune response; inflammatory response; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of cell proliferation; negative regulation of chronic inflammatory response to antigenic stimulus; negative regulation of cytokine production; negative regulation of cytokine secretion during immune response; negative regulation of heterotypic cell-cell adhesion; negative regulation of inflammatory response; negative regulation of interferon-gamma production; negative regulation of interleukin-12 production; negative regulation of interleukin-6 production; negative regulation of membrane protein ectodomain proteolysis; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of tumor necrosis factor biosynthetic process; negative regulation of tumor necrosis factor production; positive regulation of B cell apoptosis; positive regulation of cell cycle; positive regulation of cytokine secretion; positive regulation of endothelial cell proliferation; positive regulation of JAK-STAT cascade; positive regulation of macrophage activation; positive regulation of MHC class II biosynthetic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; receptor biosynthetic process; regulation of gene expression; regulation of sensory perception of pain; regulation of synapse organization and biogenesis; response to glucocorticoid stimulus; response to molecule of bacterial origin