Nectin-2 Active Protein | NECTIN2 active protein

Nectin-2 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122682

• Gene Names: Nectin2; MPH; Pvr; Pvs; Cd112; Pvrl2; AI325026; AI987993; nectin-2
• Purity: >98% as determined by SDS-PAGE
• Synonyms: Nectin-2; Nectin-2 Protein; Mouse; Recombinant (His Tag); Mouse CD112/Nectin-2/PVRL2 Protein (His Tag); AI325026 Protein; AI987993 Protein; Cd112 Protein; MPH Protein; nectin-2 Protein; Pvr Protein; Pvs Protein; poliovirus receptor-related 2 (herpesvirus entry mediator B); NECTIN2 active protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly351

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 32
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse PVRL2 (NP_033016.3) extracellular domain (Met 1-Gly 351) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA.; 1. Immobilized mouse CD112-His at 10ug/ml (100uL/well) can bind biotinylated mouse DNAM1-His with a linear range of 0.156-5.0ug/ml.; 2. Immobilized mouse CD112-His at 10ug/ml (100uL/well) can bind mouse DNAM1-Fc with a linear range of 0.03-1.0ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NECTIN2 active protein

Background: Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8⁺ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.

References

Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.

NCBI and Uniprot Product Information

• NCBI GI #: 228480273
• NCBI GeneID: 19294
• NCBI Accession #: NP_033016.3
• NCBI GenBank Nucleotide #: NM_008990.3
• UniProt Accession #: P32507; Q62096
• Molecular Weight: 50,779 Da
• NCBI Official Full Name: nectin-2 isoform 1
• NCBI Official Synonym Full Names: nectin cell adhesion molecule 2
• NCBI Official Symbol: Nectin2
• NCBI Official Synonym Symbols: MPH; Pvr; Pvs; Cd112; Pvrl2; AI325026; AI987993; nectin-2
• NCBI Protein Information: nectin-2
• UniProt Protein Name: Nectin-2
• Protein Family: Nectin
• UniProt Gene Name: Pvrl2
• UniProt Synonym Gene Names: Mph; Pvr; Pvs; Herpesvirus entry mediator B; HveB; mHveB
• UniProt Entry Name: PVRL2_MOUSE

Uniprot Description

nectin 2: Probable cell adhesion protein. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Cellular Component: focal adhesion; cell surface; membrane; cell; plasma membrane; integral to membrane; zonula adherens; intercellular junction

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; cell adhesion molecule binding

Biological Process: establishment of mitochondrion localization; acrosome formation; sperm mitochondrion organization and biogenesis; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of immunoglobulin mediated immune response; spermatid nuclear differentiation; cytoskeleton organization and biogenesis; susceptibility to natural killer cell mediated cytotoxicity; virion attachment, binding of host cell surface coreceptor; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of mast cell activation; viral envelope fusion with host membrane; fertilization; cell part morphogenesis; adhesion to host; cell adhesion; homophilic cell adhesion; spermatid development