Typical Testing Data/Standard Curve
Human Interferon epsilon (IFNE) ELISA Kit | IFNE elisa kit
Human Interferon epsilon (IFNE) ELISA Kit
Gene Names
IFNE; IFN-E; IFNE1; IFNT1; INFE1; PRO655
Reactivity
Human
Synonyms
Interferon epsilon (IFNE); Human Interferon epsilon (IFNE) ELISA Kit; IFNE elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
50-1000pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for IFNE elisa kit
Intended Uses: This IFNepsilon ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IFNepsilon. This ELISA kit for research use only!
Principle of the Assay||IFNepsilon ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IFNepsilon antibody and an IFNepsilon-HRP conjugate. The assay sample and buffer are incubated together with IFNepsilon-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNepsilon concentration since IFNepsilon from samples and IFNepsilon-HRP conjugate compete for the anti-IFNepsilon antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNepsilon from the sample, fewer sites are left to bind IFNepsilon-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNepsilon concentration in each sample is interpolated from this standard curve.
Principle of the Assay||IFNepsilon ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IFNepsilon antibody and an IFNepsilon-HRP conjugate. The assay sample and buffer are incubated together with IFNepsilon-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNepsilon concentration since IFNepsilon from samples and IFNepsilon-HRP conjugate compete for the anti-IFNepsilon antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNepsilon from the sample, fewer sites are left to bind IFNepsilon-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNepsilon concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IFNE elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,414 Da
NCBI Official Full Name
interferon epsilon
NCBI Official Synonym Full Names
interferon, epsilon
NCBI Official Symbol
IFNE
NCBI Official Synonym Symbols
IFN-E; IFNE1; IFNT1; INFE1; PRO655
NCBI Protein Information
interferon epsilon; IFN-epsilon; interferon tau-1; interferon-epsilon; interferon epsilon 1; interferon epsilon-1
UniProt Protein Name
Interferon epsilon
Protein Family
UniProt Gene Name
IFNE
UniProt Synonym Gene Names
IFNE1; UNQ360/PRO655; IFN-epsilon
UniProt Entry Name
IFNE_HUMAN
Uniprot Description
IFNE1: Belongs to the alpha/beta interferon family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9p21.3
Cellular Component: extracellular space
Molecular Function: interferon-alpha/beta receptor binding; cytokine activity
Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; B cell differentiation; defense response to bacterium; natural killer cell activation during immune response; innate immune response; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein
Research Articles on IFNE
Similar Products
Product Notes
The Human IFNE ifne (Catalog #AAA7215670) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7215670 ELISA Kit recognizes Human IFNE. It is sometimes possible for the material contained within the vial of "Interferon epsilon (IFNE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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