Mouse Interferon Regulatory Factor 3 (IRF3) ELISA Kit | IRF3 elisa kit

Mouse Interferon Regulatory Factor 3 (IRF3) ELISA Kit

Cat. Num. AAA453282

• Gene Names: Irf3; IRF-3; C920001K05Rik
• Reactivity: Mouse
• Synonyms: Interferon Regulatory Factor 3 (IRF3); Mouse Interferon Regulatory Factor 3 (IRF3) ELISA Kit; IRF3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of IRF3. No significant cross-reactivity or interference between IRF3 and analogues was observed.
• Sequence Length: 419

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.048ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IRF3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IRF3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IRF3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IRF3 in mouse tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IRF3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IRF3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IRF3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IRF3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8393627
• NCBI GeneID: 54131
• NCBI Accession #: NP_058545.1
• NCBI GenBank Nucleotide #: NM_016849.4
• UniProt Accession #: P70671
• Molecular Weight: 46,852 Da
• NCBI Official Full Name: interferon regulatory factor 3
• NCBI Official Synonym Full Names: interferon regulatory factor 3
• NCBI Official Symbol: Irf3
• NCBI Official Synonym Symbols: IRF-3; C920001K05Rik
• NCBI Protein Information: interferon regulatory factor 3
• UniProt Protein Name: Interferon regulatory factor 3
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: Irf3
• UniProt Synonym Gene Names: IRF-3

Uniprot Description

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.