Mouse Insulin Receptor Substrate 2 (IRS2) ELISA Kit | IRS2 elisa kit

Mouse Insulin Receptor Substrate 2 (IRS2) ELISA Kit

Cat. Num. AAA2705286

• Gene Names: IRS2; IRS-2
• Reactivity: Mouse
• Synonyms: Insulin Receptor Substrate 2 (IRS2); Mouse Insulin Receptor Substrate 2 (IRS2) ELISA Kit; IRS2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Insulin Receptor Substrate 2 (IRS2).; No significant cross-reactivity or interference between Insulin Receptor Substrate 2 (IRS2) and analogues was observed.
• Sequence Length: 1,338

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.053ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Receptor Substrate 2 (IRS2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Receptor Substrate 2 (IRS2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IRS2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Receptor Substrate 2 (IRS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Receptor Substrate 2 (IRS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Receptor Substrate 2 (IRS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Receptor Substrate 2 (IRS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 38683860
• NCBI GeneID: 8660
• NCBI Accession #: NP_003740.2
• NCBI GenBank Nucleotide #: NM_003749.2
• UniProt Accession #: Q9Y4H2; Q96RR2; Q9BZG0; Q9Y6I5
• Molecular Weight: 137,334 Da
• NCBI Official Full Name: insulin receptor substrate 2
• NCBI Official Synonym Full Names: insulin receptor substrate 2
• NCBI Official Symbol: IRS2
• NCBI Official Synonym Symbols: IRS-2
• NCBI Protein Information: insulin receptor substrate 2
• UniProt Protein Name: Insulin receptor substrate 2
• Protein Family: Insulin receptor substrate
• UniProt Gene Name: IRS2
• UniProt Synonym Gene Names: IRS-2

NCBI Description

This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq, Jul 2008]

Uniprot Description

May mediate the control of various cellular processes by insulin.