Sheep Insulin receptor substrate 2 ELISA Kit | IRS-2 elisa kit

Sheep Insulin receptor substrate 2 ELISA Kit

Cat. Num. AAA747086

• Gene Names: IRS2; IRS-2
• Reactivity: Sheep
• Synonyms: Insulin receptor substrate 2; Sheep Insulin receptor substrate 2 ELISA Kit; IRS-2 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Competitive (Quantitative)
• Sensitivity: 1.0 pg/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IRS-2 elisa kit

Intended Uses: This IRS2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep IRS2. This ELISA kit for research use only!

Principle of the Assay: IRS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IRS2 antibody and an IRS2-HRP conjugate. The assay sample and buffer are incubated together with IRS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IRS2 concentration since IRS2 from samples and IRS2-HRP conjugate compete for the anti-IRS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IRS2 from the sample, fewer sites are left to bind IRS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IRS2 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 38683860
• NCBI GeneID: 8660
• NCBI Accession #: NP_003740.2
• NCBI GenBank Nucleotide #: NM_003749.2
• Molecular Weight: 137,334 Da
• NCBI Official Full Name: insulin receptor substrate 2
• NCBI Official Synonym Full Names: insulin receptor substrate 2
• NCBI Official Symbol: IRS2
• NCBI Official Synonym Symbols: IRS-2
• NCBI Protein Information: insulin receptor substrate 2
• UniProt Protein Name: Insulin receptor substrate 2
• Protein Family: Insulin receptor substrate
• UniProt Gene Name: IRS2
• UniProt Synonym Gene Names: IRS-2
• UniProt Entry Name: IRS2_HUMAN

NCBI Description

This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq, Jul 2008]

Uniprot Description

IRS2: May mediate the control of various cellular processes by insulin. Interacts with PHIP.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: plasma membrane; cytosol

Molecular Function: protein domain specific binding; signal transducer activity; protein binding; phosphoinositide 3-kinase binding; protein kinase binding; protein phosphatase binding; insulin receptor binding

Biological Process: negative regulation of kinase activity; regulation of lipid metabolic process; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; lipid homeostasis; signal transduction; positive regulation of glucose import; mammary gland development; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; response to glucose stimulus; positive regulation of B cell proliferation; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; glucose metabolic process; positive regulation of insulin secretion; positive regulation of fatty acid beta-oxidation; cell proliferation; cellular response to insulin stimulus; insulin receptor signaling pathway; innate immune response; brain development; negative regulation of B cell apoptosis; positive regulation of cell migration

Disease: Diabetes Mellitus, Noninsulin-dependent