Human Insulin receptor substrate 2 ELISA Kit | IRS2 elisa kit

Human Insulin receptor substrate 2 ELISA Kit

Cat. Num. AAA763553

• Gene Names: IRS2; IRS-2
• Reactivity: Human
• Synonyms: Insulin receptor substrate 2; Human Insulin receptor substrate 2 ELISA Kit; IRS2 (Insulin receptor substrate 2)/IRS-2; IRS2 elisa kit

• Reactivity: Human
• Sequence Length: 1338

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for IRS2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human IRS2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human IRS2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IRS2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human IRS2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 38683860
• NCBI GeneID: 8660
• NCBI Accession #: NP_003740.2
• NCBI GenBank Nucleotide #: NM_003749.2
• Molecular Weight: 137,334 Da
• NCBI Official Full Name: insulin receptor substrate 2
• NCBI Official Synonym Full Names: insulin receptor substrate 2
• NCBI Official Symbol: IRS2
• NCBI Official Synonym Symbols: IRS-2
• NCBI Protein Information: insulin receptor substrate 2
• UniProt Protein Name: Insulin receptor substrate 2
• Protein Family: Insulin receptor substrate
• UniProt Gene Name: IRS2
• UniProt Synonym Gene Names: IRS-2
• UniProt Entry Name: IRS2_HUMAN

NCBI Description

This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq, Jul 2008]

Uniprot Description

IRS2: May mediate the control of various cellular processes by insulin. Interacts with PHIP.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: cytosol; plasma membrane

Molecular Function: insulin receptor binding; phosphoinositide 3-kinase binding; protein binding; protein domain specific binding; protein kinase binding; protein phosphatase binding; signal transducer activity

Biological Process: activation of MAPKK activity; axon guidance; brain development; cell proliferation; cellular response to insulin stimulus; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; glucose metabolic process; innate immune response; insulin receptor signaling pathway; lipid homeostasis; mammary gland development; MAPKKK cascade; negative regulation of B cell apoptosis; negative regulation of kinase activity; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of B cell proliferation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of fatty acid beta-oxidation; positive regulation of glucose import; positive regulation of glycogen biosynthetic process; positive regulation of insulin secretion; positive regulation of mesenchymal cell proliferation; Ras protein signal transduction; regulation of lipid metabolic process; response to glucose stimulus; signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway

Disease: Diabetes Mellitus, Noninsulin-dependent