Mouse Anti-Mullerian Hormone (AMH) ELISA Kit | AMH elisa kit

Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) ELISA Kit

Cat. Num. AAA2709333

• Gene Names: Amh; MIS
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Anti-Mullerian Hormone (AMH); Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) ELISA Kit; MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance; AMH elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Anti-AMH. No significant cross-reactivity or interference between Anti-AMH and analogues was observed.

• Applicable Applications for AMH elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antibody Detection.
• Samples: Serum, plasma and other biological fluids
• Detection Range: 3.12-200ng/mL
• Sensitivity: < 1.19ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-AMH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-AMH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AMH elisa kit

Intended Uses: The kit is an enzyme immunoassay for in vitro quantitative measurement of Anti-AMH in mouse serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with AMH. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-AMH will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-AMH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 127110
• NCBI GeneID: 11705
• UniProt Accession #: P27106
• Molecular Weight: 59,778 Da
• NCBI Official Full Name: Muellerian-inhibiting factor
• NCBI Official Synonym Full Names: anti-Mullerian hormone
• NCBI Official Symbol: Amh
• NCBI Official Synonym Symbols: MIS
• NCBI Protein Information: muellerian-inhibiting factor; anti-Muellerian hormone; Mullerian inhibiting substance; muellerian-inhibiting substance
• UniProt Protein Name: Muellerian-inhibiting factor
• Protein Family: Muellerian-inhibiting factor
• UniProt Gene Name: Amh
• UniProt Synonym Gene Names: AMH; MIS
• UniProt Entry Name: MIS_MOUSE

NCBI Description

This gene is a member of the transforming growth factor-beta superfamily of growth and differentiation factors. The encoded protein is produced in fetal Sertoli cells during male gonadal differentiation. The protein binds to the anti-Mullerian hormone receptor type 2 and causes the regression of Mullerian ducts in developing male embryos. In humans, some mutations in this gene are associated with persistent Mullerian duct syndrome (PMDS). [provided by RefSeq, Apr 2013]

Uniprot Description

Function: This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.

Subunit structure: Homodimer; disulfide-linked.

Subcellular location: Secreted

By similarity.

Tissue specificity: Sertoli cells of fetal testes, and testes just after birth, but absent in adult testes. In female, AMH is expressed after birth in the granulosa cells of the follicle. AMH expression is dependent on the degree of follicular maturation and not on the age of the ovary.

Miscellaneous: Although it does not compete with EGF for receptor binding sites, MIS can inhibit the autophosphorylation of the EGF receptor in vitro.

Sequence similarities: Belongs to the TGF-beta family.