Mouse IL-1alpha (Interleukin 1Alpha) ELISA Kit | IL-1alpha elisa kit

Mouse IL-1alpha (Interleukin 1Alpha) ELISA Kit

Cat. Num. AAA2568054

• Gene Names: Il1a; Il-1a
• Reactivity: Mouse
• Synonyms: IL-1alpha (Interleukin 1Alpha); Mouse IL-1alpha (Interleukin 1Alpha) ELISA Kit; IL1A; IL1-A; IL1; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; IL-1alpha; IL-1alpha elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes Mouse IL-1alpha in samples. No significant cross-reactivity or interference between Mouse IL-1alpha and analogues was observed.; This ELISA kit applies to the in vitro quantitative determination of Mouse IL-1alpha concentrations in serum, plasma and other biological fluids.

• Assay Type: Sandwich ELISA
• Detection Method: Colormetric
• Tested Sample Types: serum, plasma and other biological fluids
• Detection Range: 0.78~50pg/mL
• Sensitivity: 0.47pg/mL
• Precision: Both intra-CV and inter-CV are < 10%.
• Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm +/- 2nm. The OD value is proportional to the concentration of Mouse IL-1alpha. You can calculate the concentration of Mouse IL-1alpha in the samples by comparing the OD of the samples to the standard curve.
• Preparation and Storage: An unopened kit can be stored at 2-8 degree C for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the *following conditions (manual) once the kit is received.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 47059075
• NCBI GeneID: 16175
• NCBI Accession #: NP_034684.2
• NCBI GenBank Nucleotide #: NM_010554.4
• UniProt Accession #: P01582
• Molecular Weight: 31,023 Da
• NCBI Official Full Name: interleukin-1 alpha
• NCBI Official Synonym Full Names: interleukin 1 alpha
• NCBI Official Symbol: Il1a
• NCBI Official Synonym Symbols: Il-1a
• NCBI Protein Information: interleukin-1 alpha
• UniProt Protein Name: Interleukin-1 alpha
• UniProt Gene Name: Il1a
• UniProt Synonym Gene Names: IL-1 alpha
• UniProt Entry Name: IL1A_MOUSE

Uniprot Description

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family.

Protein type: Cytokine; Motility/polarity/chemotaxis

Cellular Component: cell surface; cytoplasm; cytosol; extracellular space; plasma membrane

Molecular Function: copper ion binding; cytokine activity; interleukin-1 receptor binding

Biological Process: connective tissue replacement during inflammatory response; cytokine and chemokine mediated signaling pathway; germ cell programmed cell death; inflammatory response; keratinization; negative regulation of cell proliferation; positive regulation of angiogenesis; positive regulation of cytokine secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of mitosis; positive regulation of prostaglandin secretion; positive regulation of protein secretion; positive regulation of stress-activated MAPK cascade; positive regulation of transcription from RNA polymerase II promoter; regulation of actin cytoskeleton organization and biogenesis; regulation of sensory perception of pain; response to copper ion