Mouse Interleukin 1 Alpha (IL1a) RTU ELISA Kit | IL1a rtu elisa kit

Mouse Interleukin 1 Alpha (IL1a) ELISA Kit

Cat. Num. AAA4500262

• Gene Names: Il1a; Il-1a
• Reactivity: Mouse
• Synonyms: Interleukin 1 Alpha (IL1a); Mouse Interleukin 1 Alpha (IL1a) ELISA Kit; IL1-A; IL-1alpha; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1; IL1a rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 270

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 5.9pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL1a rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL1a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IL1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47059075
• NCBI GeneID: 16175
• NCBI Accession #: NP_034684.2
• NCBI GenBank Nucleotide #: NM_010554.4
• UniProt Accession #: P01582
• Molecular Weight: 31,023 Da
• NCBI Official Full Name: interleukin-1 alpha
• NCBI Official Synonym Full Names: interleukin 1 alpha
• NCBI Official Symbol: Il1a
• NCBI Official Synonym Symbols: Il-1a
• NCBI Protein Information: interleukin-1 alpha
• UniProt Protein Name: Interleukin-1 alpha
• Protein Family: Interleukin
• UniProt Gene Name: Il1a
• UniProt Synonym Gene Names: IL-1 alpha

Uniprot Description

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.