Mouse EPO (Erythropoietin) ELISA Kit | EPO elisa kit

Mouse EPO (Erythropoietin) ELISA Kit

Cat. Num. AAA2568053

• Reactivity: Mouse
• Synonyms: EPO (Erythropoietin); Mouse EPO (Erythropoietin) ELISA Kit; EP; MVCD2; Epoetin; Hematopoietin; Hemopoietin; EPO elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes Mouse EPO in samples. No significant cross-reactivity or interference between Mouse EPO and analogues was observed.; This ELISA kit applies to the in vitro quantitative determination of Mouse EPO concentrations in serum, plasma and other biological fluids.

• Assay Type: Sandwich ELISA
• Detection Method: Colormetric
• Tested Sample Types: Serum, plasma and other biological fluids
• Detection Range: 7.81~500pg/mL
• Sensitivity: 4.69pg/mL
• Precision: Both intra-CV and inter-CV are < 10%.
• Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse EPO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse EPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm +/- 2nm. The OD value is proportional to the concentration of Mouse EPO. You can calculate the concentration of Mouse EPO in the samples by comparing the OD of the samples to the standard curve.
• Preparation and Storage: An unopened kit can be stored at 2-8 degree C for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the *following conditions (manual) once the kit is received.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 119528
• NCBI GeneID: 13856
• UniProt Accession #: P07321
• Molecular Weight: 21,365 Da
• NCBI Official Full Name: Erythropoietin
• NCBI Official Synonym Full Names: erythropoietin
• NCBI Official Symbol: Epo
• NCBI Protein Information: erythropoietin
• UniProt Protein Name: Erythropoietin
• Protein Family: Erythropoietin
• UniProt Gene Name: Epo
• UniProt Entry Name: EPO_MOUSE

NCBI Description

This gene encodes the glycoprotein hormone erythropoietin that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults. A complete lack of the encoded protein causes embryonic lethal anemia in mice. The conditional inactivation of this gene in adult mice results in a chronic, normocytic and normochromic anemia. Transgenic mice expressing the human ortholog of this gene exhibit polycythemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

Uniprot Description

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: erythropoietin receptor binding; hormone activity; protein kinase activator activity

Biological Process: hemoglobin biosynthetic process; heart morphogenesis; erythrocyte maturation; apoptosis; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; negative regulation of myeloid cell apoptosis; peptidyl-serine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of protein kinase activity; positive regulation of cell proliferation; cardiac muscle morphogensis; response to hypoxia; erythrocyte differentiation; ventricular cardiac muscle morphogenesis; angiogenesis; positive regulation of Ras protein signal transduction; positive regulation of neuron differentiation; embryo implantation; positive regulation of DNA replication; negative regulation of apoptosis