Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Histone deacetylase complex subunit SAP130 (SAP130) Standard Curve.)
Mouse Histone Deacetylase Complex Subunit SAP130 (SAP130) ELISA Kit | SAP130 elisa kit
Mouse Histone Deacetylase Complex Subunit SAP130 (SAP130) ELISA Kit
Gene Names
Sap130; 6720406D06; 2610304F09Rik
Reactivity
Mouse
Synonyms
Histone Deacetylase Complex Subunit SAP130 (SAP130); Mouse Histone Deacetylase Complex Subunit SAP130 (SAP130) ELISA Kit; SAP130; FLJ12761; Sin3-associated polypeptide p130; histone deacetylase complex subunit SAP130; mSin3A-associated protein 130; SAP130 elisa kit
Reactivity
Mouse
Specificity
Mouse Histone deacetylase complex subunit SAP130 (SAP130) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SAP130. No significant cross-reactivity or interference between Mouse SAP130 and analogues was observed.
Sequence Length
1056
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Histone deacetylase complex subunit SAP130 (SAP130) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Histone deacetylase complex subunit SAP130 (SAP130) Standard Curve.)
Related Product Information for SAP130 elisa kit
Description: This Mouse Histone deacetylase complex subunit SAP130 (SAP130) ELISA Kit employs a two-site sandwich ELISA to quantitate SAP130 in samples. An antibody specific for SAP130 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SAP130 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SAP130 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SAP130 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SF3B3 encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.
Background: SF3B3 encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
histone deacetylase complex subunit SAP130 isoform 1
NCBI Official Synonym Full Names
Sin3A associated protein
NCBI Official Symbol
Sap130
NCBI Official Synonym Symbols
6720406D06; 2610304F09Rik
NCBI Protein Information
histone deacetylase complex subunit SAP130
UniProt Protein Name
Histone deacetylase complex subunit SAP130
Protein Family
UniProt Gene Name
Sap130
UniProt Entry Name
SP130_MOUSE
Uniprot Description
SAP130: Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. Belongs to the SAP130 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; Acetyltransferase
Cellular Component: nucleoplasm; nucleus
Molecular Function: protein binding; histone acetyltransferase activity; transcription coactivator activity
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
Research Articles on SAP130
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Product Notes
The Mouse SAP130 sap130 (Catalog #AAA9717087) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717087 ELISA Kit recognizes Mouse SAP130. It is sometimes possible for the material contained within the vial of "Histone Deacetylase Complex Subunit SAP130 (SAP130), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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