Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Small nuclear ribonucleoproteinantibody (SNRNP-Ab) Standard Curve.)
Human Small Nuclear Ribonucleoproteinantibody (SNRNP-Ab) ELISA Kit | SNRNP-Ab elisa kit
Human Small Nuclear Ribonucleoproteinantibody (SNRNP-Ab) ELISA Kit
Reactivity
Human
Synonyms
Small Nuclear Ribonucleoproteinantibody (SNRNP-Ab); Human Small Nuclear Ribonucleoproteinantibody (SNRNP-Ab) ELISA Kit; SNRNP-Ab; SM; SNRNP-Ab elisa kit
Reactivity
Human
Specificity
Human Small nuclear ribonucleoproteinantibody (SNRNP-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human SNRNP-Ab. No significant cross-reactivity or interference between Human SNRNP-Ab and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Small nuclear ribonucleoproteinantibody (SNRNP-Ab) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Small nuclear ribonucleoproteinantibody (SNRNP-Ab) Standard Curve.)
Related Product Information for SNRNP-Ab elisa kit
Description: This Human Small nuclear ribonucleoproteinantibody (SNRNP-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate SNRNP-Ab in samples. An antibody specific for SNRNP-Ab has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SNRNP-Ab present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SNRNP-Ab is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SNRNP-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: snRNPs (pronounced "snurps"), or small nuclear ribonucleo proteins, are RNA-protein complexes that combine with unmodified pre-mRNA and various other proteins to form a spliceosome, a large RNA-protein molecular complex upon which splicing of pre-mRNA occurs. The action of snRNPs is essential to the removal of introns from pre-mRNA, a critical aspect of post-transcriptional modification of RNA, occurring only in the nucleus of eukaryotic cells. Additionally, U7 snRNP is not involved in splicing at all, as U7 snRNP is responsible for processing the 3? stem-loop of histone pre-mRNA. The two essential components of snRNPs are protein molecules and RNA. The RNA found within each snRNP particle is known as small nuclear RNA, or snRNA, and is usually about 150 nucleotides in length. The snRNA component of the snRNP gives specificity to individual introns by "recognizing" the sequences of critical splicing signals at the 5' and 3' ends and branch site of introns. The snRNA in snRNPs is similar to ribosomal RNA in that it directly incorporates both an enzymatic and a structural role. SnRNPs were discovered by Michael R. Lerner and Joan A. Steitz. Thomas R. Cech and Sidney Altman also played a role in the discovery, winning the Nobel Prize for Chemistry in 1989 for their independent discoveries that RNA can act as a catalyst in cell development.
Background: snRNPs (pronounced "snurps"), or small nuclear ribonucleo proteins, are RNA-protein complexes that combine with unmodified pre-mRNA and various other proteins to form a spliceosome, a large RNA-protein molecular complex upon which splicing of pre-mRNA occurs. The action of snRNPs is essential to the removal of introns from pre-mRNA, a critical aspect of post-transcriptional modification of RNA, occurring only in the nucleus of eukaryotic cells. Additionally, U7 snRNP is not involved in splicing at all, as U7 snRNP is responsible for processing the 3? stem-loop of histone pre-mRNA. The two essential components of snRNPs are protein molecules and RNA. The RNA found within each snRNP particle is known as small nuclear RNA, or snRNA, and is usually about 150 nucleotides in length. The snRNA component of the snRNP gives specificity to individual introns by "recognizing" the sequences of critical splicing signals at the 5' and 3' ends and branch site of introns. The snRNA in snRNPs is similar to ribosomal RNA in that it directly incorporates both an enzymatic and a structural role. SnRNPs were discovered by Michael R. Lerner and Joan A. Steitz. Thomas R. Cech and Sidney Altman also played a role in the discovery, winning the Nobel Prize for Chemistry in 1989 for their independent discoveries that RNA can act as a catalyst in cell development.
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Product Notes
The Human SNRNP-Ab (Catalog #AAA9715790) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715790 ELISA Kit recognizes Human SNRNP-Ab. It is sometimes possible for the material contained within the vial of "Small Nuclear Ribonucleoproteinantibody (SNRNP-Ab), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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