Western Blot (WB)
(Western blot analysis of extracts of mouse brain, using SAP130 antibody.)
Rabbit anti-Mouse SAP130 Polyclonal Antibody | anti-SAP130 antibody
SAP130 Polyclonal Antibody
Reactivity
Mouse
Applications
Western Blot
Purity
Affinity Purification
Synonyms
SAP130; Polyclonal Antibody; SAP130 Polyclonal Antibody; 130 kDa Sin3-associated polypeptide; FLJ12761; Histone deacetylase complex subunit SAP130; KIAA0017; mSin3A associated protein 130; Pre mRNA splicing factor SF3b 130 kDa subunit; RSE1; SAP 130; Sap130; SF3b 130; SF3b130; SF3B3; Sin3-associated polypeptide p130; Sin3A associated protein 130kDa; Sin3A associated protein; SP130; Spliceosome associated protein 130; Splicing factor 3b subunit 3 130kDa; Splicing factor 3b subunit 3; STAF 130; STAF130; anti-SAP130 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration
1 mg/mL (varies by lot)
Sequence Length
613
Applicable Applications for anti-SAP130 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant protein of human SAP130
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of mouse brain, using SAP130 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of mouse brain, using SAP130 antibody.)
Related Product Information for anti-SAP130 antibody
SF3b is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3b contains the spliceosomal proteins SAP 49, SAP 130, SAP 145, and SAP 155. SAP 130, SAP 145, and SAP 155 are present in a protein complex and associate with one another. While SAP 155 and SAP 130 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 65kDa; 110kDa; 113kDa
Observed: 150kDa
Observed: 150kDa
NCBI Official Full Name
histone deacetylase complex subunit SAP130 isoform a
NCBI Official Synonym Full Names
Sin3A associated protein 130
NCBI Official Symbol
SAP130
NCBI Protein Information
histone deacetylase complex subunit SAP130
UniProt Protein Name
Histone deacetylase complex subunit SAP130
Protein Family
UniProt Gene Name
SAP130
NCBI Description
SAP130 is a subunit of the histone deacetylase (see HDAC1; MIM 601241)-dependent SIN3A (MIM 607776) corepressor complex (Fleischer et al., 2003 [PubMed 12724404]).[supplied by OMIM, Mar 2008]
Uniprot Description
Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
Research Articles on SAP130
Similar Products
Product Notes
The SAP130 sap130 (Catalog #AAA2555231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SAP130 Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SAP130 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the SAP130 sap130 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAP130, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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