Western Blot (WB)
(staining (0.1ug/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat SAP130 Polyclonal Antibody
SAP130 (Spliceosome-associated Protein 130, SAP 130, KIAA0017, Pre-mRNA-splicing Factor SF3b 130kD Subunit, RSE1, Splicing Factor 3B Subunit 3, SF3B3, SF3b130, STAF130)
Reactivity
Bovine, Canine, Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
SAP130; Polyclonal Antibody; SAP130 (Spliceosome-associated Protein 130; SAP 130; KIAA0017; Pre-mRNA-splicing Factor SF3b 130kD Subunit; RSE1; Splicing Factor 3B Subunit 3; SF3B3; SF3b130; STAF130); Anti -SAP130 (Spliceosome-associated Protein 130; anti-SAP130 antibody
Host
Goat
Reactivity
Bovine, Canine, Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes human SAP130. Species sequence homology: Bovine, canine, mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Applicable Applications for anti-SAP130 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:2000
Western Blot: 0.1-0.3ug/ml, nuclear lysates of cell line HeLa observed on ~150kD bands
Dilution: ELISA: 1:2000
Western Blot: 0.1-0.3ug/ml, nuclear lysates of cell line HeLa observed on ~150kD bands
Immunogen
Synthetic peptide corresponding to C-VSKKLEDIRTRYAF, from human SAP130, at C-terminal (NP_036558.3).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(staining (0.1ug/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(staining (0.1ug/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Related Product Information for anti-SAP130 antibody
This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.
Product Categories/Family for anti-SAP130 antibody
Similar Products
Product Notes
The SAP130 (Catalog #AAA646123) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The SAP130 (Spliceosome-associated Protein 130, SAP 130, KIAA0017, Pre-mRNA-splicing Factor SF3b 130kD Subunit, RSE1, Splicing Factor 3B Subunit 3, SF3B3, SF3b130, STAF130) reacts with Bovine, Canine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SAP130 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:2000 Western Blot: 0.1-0.3ug/ml, nuclear lysates of cell line HeLa observed on ~150kD bands. Researchers should empirically determine the suitability of the SAP130 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAP130, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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