Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse GRB2-associated-binding protein 2 (GAB2) Standard Curve.)
Mouse GRB2-Associated-Binding Protein 2 (GAB2) ELISA Kit | GAB2 elisa kit
Mouse GRB2-Associated-Binding Protein 2 (GAB2) ELISA Kit
Gene Names
Gab2; p97; AI463667; D130058I17Rik
Reactivity
Mouse
Synonyms
GRB2-Associated-Binding Protein 2 (GAB2); Mouse GRB2-Associated-Binding Protein 2 (GAB2) ELISA Kit; GAB2; KIAA0571; Grb2-associated binder 2; growth factor receptor bound protein 2-associated protein 2; GAB2 elisa kit
Reactivity
Mouse
Specificity
Mouse GRB2-associated-binding protein 2 (GAB2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse GAB2. No significant cross-reactivity or interference between Mouse GAB2 and analogues was observed.
Sequence Length
665
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse GRB2-associated-binding protein 2 (GAB2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse GRB2-associated-binding protein 2 (GAB2) Standard Curve.)
Related Product Information for GAB2 elisa kit
Description: This Mouse GRB2-associated-binding protein 2 (GAB2) ELISA Kit employs a two-site sandwich ELISA to quantitate GAB2 in samples. An antibody specific for GAB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: GAB2 is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. In contrast to the reciprocal effects of GAB1 and GAB2 in mediating ELK1 induction, these 2 molecules have a similar function in extracellular signal-regulated kinase activation induced by either oncogenic Ras or growth factor stimulation.
Background: GAB2 is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. In contrast to the reciprocal effects of GAB1 and GAB2 in mediating ELK1 induction, these 2 molecules have a similar function in extracellular signal-regulated kinase activation induced by either oncogenic Ras or growth factor stimulation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
GRB2-associated-binding protein 2
NCBI Official Synonym Full Names
growth factor receptor bound protein 2-associated protein 2
NCBI Official Symbol
Gab2
NCBI Official Synonym Symbols
p97; AI463667; D130058I17Rik
NCBI Protein Information
GRB2-associated-binding protein 2
UniProt Protein Name
GRB2-associated-binding protein 2
Protein Family
UniProt Gene Name
Gab2
UniProt Entry Name
GAB2_MOUSE
Research Articles on GAB2
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Product Notes
The Mouse GAB2 gab2 (Catalog #AAA9718093) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718093 ELISA Kit recognizes Mouse GAB2. It is sometimes possible for the material contained within the vial of "GRB2-Associated-Binding Protein 2 (GAB2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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