Mouse Free Fatty Acid (FFA) ELISA Kit | FFA elisa kit

Mouse Free Fatty Acid (FFA) ELISA Kit

Cat. Num. AAA265030

• Gene Names: FFAR2; FFA2R; GPR43
• Reactivity: Mouse
• Synonyms: Free Fatty Acid (FFA); Mouse Free Fatty Acid (FFA) ELISA Kit; FFA elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 330

• Samples: Mouse serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 mmol/L-0.156 mmol/L
• Sensitivity: Up to 0.05 mmol/L
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FFA elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse FFA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4885333
• NCBI GeneID: 2867
• NCBI Accession #: NP_005297.1
• NCBI GenBank Nucleotide #: NM_005306.2
• Molecular Weight: 37,144 Da
• NCBI Official Full Name: free fatty acid receptor 2
• NCBI Official Synonym Full Names: free fatty acid receptor 2
• NCBI Official Symbol: FFAR2
• NCBI Official Synonym Symbols: FFA2R; GPR43
• NCBI Protein Information: free fatty acid receptor 2; G protein-coupled receptor 43; G-protein coupled receptor 43; free fatty acid activated receptor 2
• UniProt Protein Name: Free fatty acid receptor 2
• UniProt Gene Name: FFAR2
• UniProt Synonym Gene Names: GPR43
• UniProt Entry Name: FFAR2_HUMAN

NCBI Description

This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for short chain free fatty acids and may be involved in the inflammatory response and in regulating lipid plasma levels. [provided by RefSeq, Apr 2009]

Uniprot Description

FFAR2: Receptor for short chain fatty acids through a G(i)- protein-mediated inhibition of adenylyl cyclase and elevation of intracellular calcium. The rank order of potency for agonists of this receptor is acetate= propionate = butyrate > pentanoate = formate. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cell projection; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; lipid binding

Biological Process: fat cell differentiation; G-protein coupled receptor protein signaling pathway; sequestering of lipid; mucosal immune response; regulation of acute inflammatory response; positive regulation of cytokine production during immune response; cell surface pattern recognition receptor signaling pathway; positive regulation of acute inflammatory response to non-antigenic stimulus; glucose homeostasis; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production