MOCS2 recombinant protein

MOCS2, 1-188aa, Human, His tag, E Coli

Cat. Num. AAA204557

• Gene Names: MOCS2; MPTS; MCBPE; MOCO1; MOCODB
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: MOCS2; 1-188aa; Human; His tag; E Coli; molybdenum cofactor synthesis 2; MCBPE; MOCO1; MOCS2A; MOCS2B; MPTS; MOCS2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSSL EISSSCFSLE TKLPLSPPLV EDSAFEPSRK DMDEVEEKSK DVINFTAEKL SVDEVSQLVI SPLCGAISLF VGTTRNNFEG KKVISLEYEA YLPMAENEVR KICSDIRQKW PVKHIAVFHR LGLVPVSEAS IIIAVSSAHR AASLEAVSYA IDTLKAKVPI WKKEIYEESS TWKGNKECFW ASNS
• Sequence Length: 188

• Applicable Applications for MOCS2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for MOCS2 recombinant protein

MOCS2 is a heterotetrameric synthase composed of two small (MOCS2A) and two large (MOCS2B) subunits. The large and small subunits of molybdopterin synthase are both encoded from this gene by overlapping open reading frames. MOCS2 functions in the second step of the synthesis of molybdenum cofactor or molybdopterin (MPT). It catalyzes the formation of MPT from precursor Z by incorporating a dithiolene functional group. Recombinant human MOCS2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for MOCS2 recombinant protein

Metabolism

References

Leimkuhler S., et al. (2005) Hum Genet. 117: 565-570; Reiss J., et al. (1999) Hum Genet. 64: 706-711.

NCBI and Uniprot Product Information

• NCBI GI #: 4758732
• NCBI GeneID: 4338
• NCBI Accession #: NP_004522
• NCBI GenBank Nucleotide #: NM_004531.4
• UniProt Accession #: O96007; Q6IAI3; O96033
• Molecular Weight: 25 kDa (224aa) confirmed by MALDI-TOF
• NCBI Official Full Name: molybdopterin synthase catalytic subunit large subunit MOCS2B
• NCBI Official Synonym Full Names: molybdenum cofactor synthesis 2
• NCBI Official Symbol: MOCS2
• NCBI Official Synonym Symbols: MPTS; MCBPE; MOCO1; MOCODB
• NCBI Protein Information: molybdopterin synthase catalytic subunit; molybdopterin synthase sulfur carrier subunit
• UniProt Protein Name: Molybdopterin synthase catalytic subunit
• UniProt Gene Name: MOCS2
• UniProt Synonym Gene Names: MPT synthase large subunit

NCBI Description

Eukaryotic molybdoenzymes use a unique molybdenum cofactor (MoCo) consisting of a pterin, termed molybdopterin, and the catalytically active metal molybdenum. MoCo is synthesized from precursor Z by the heterodimeric enzyme molybdopterin synthase. The large and small subunits of molybdopterin synthase are both encoded from this gene by overlapping open reading frames. The proteins were initially thought to be encoded from a bicistronic transcript. They are now thought to be encoded from monocistronic transcripts. Alternatively spliced transcripts have been found for this locus that encode the large and small subunits. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalytic subunit of the molybdopterin synthase complex, a complex that catalyzes the conversion of precursor Z into molybdopterin. Acts by mediating the incorporation of 2 sulfur atoms from thiocarboxylated MOCS2A into precursor Z to generate a dithiolene group.