Mouse Fractalkine/CX3CL1 ELISA Kit | CX3CL1 elisa kit

Mouse Fractalkine/CX3CL1 PicoKine ELISA Kit

Cat. Num. AAA175845

• Gene Names: CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
• Reactivity: Mouse
• Synonyms: Fractalkine/CX3CL1; Mouse Fractalkine/CX3CL1 PicoKine ELISA Kit; Fractalkine; chemokine (C-X3-C motif) ligand 1; A 152E5.2; AB030188; ABCD 3; ABCD3; C X3 C motif chemokine 1; C3Xkine; Chemokine (C X3 C motif) ligand 1; Chemokine C X3 C motif ligand 1; Chemokine CX3C Motif Ligand 1; CX3C membrane anchored chemokine; CXC3; CXC3C; FKN; Neurotactin; NTN; NTT; SCYD 1; SCYD1; CX3XL1; Small inducible cytokine D1; Small inducible cytokine subfamily D (Cys X3 Cys); member 1; Small inducible cytokine subfamily D (Cys-X3-Cys); member 1 (fractalkine; neurotactin); Small inducible cytokine subfamily D member 1; CX3CL1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse Fractalkine
• Sequence Length: 397

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA) and tissue homogenates
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice).1, 2 CX3CL1 is produced as a long protein (with 373-amino acid inhumans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble (90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed.2 CX3CL1 elicits its adhesiveand migratory functions by interacting with the chemokine receptor CX3CR1.3 Its gene is located on humanchromosome 16 along with some CC chemokines known as CCL17 and CCL22.2, 4 It can act as a mediator ofsmooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.5
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse Fractalkine/CX3CL1 PicoKine ELISA Kit standard curve )

Related Product Information for CX3CL1 elisa kit

Principle of the assay: mouse Fractalkine ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Fractalkine has been precoated onto 96-well plates. Standards(sf21, Q25-R337) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fractalkine is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Fractalkine amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GeneID: 6376
• UniProt Accession #: A0N0N7; P78423
• Molecular Weight: 42,203 Da
• NCBI Official Synonym Full Names: chemokine (C-X3-C motif) ligand 1
• NCBI Official Symbol: CX3CL1
• NCBI Official Synonym Symbols: NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
• NCBI Protein Information: fractalkine; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)
• UniProt Protein Name: Chemokine (C-X3-C motif) ligand 1
• Protein Family: Fractalkine
• UniProt Gene Name: CX3CL1
• UniProt Entry Name: A0N0N7_HUMAN

Uniprot Description

CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; cell surface; extracellular region; integral to membrane; plasma membrane

Molecular Function: protein binding; chemokine activity; receptor binding

Biological Process: neutrophil chemotaxis; positive regulation of transforming growth factor-beta1 production; leukocyte chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; angiogenesis involved in wound healing; chemotaxis; macrophage chemotaxis; leukocyte adhesive activation; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of inflammatory response