Typical Testing Data/Standard Curve (for reference only)
Mouse CX3C-chemokine/Fractalkine ELISA Kit | FKN elisa kit
Mouse CX3C-chemokine/Fractalkine ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CX3C ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CX3C. Standards or samples are then added to the microtiter plate wells and CX3C if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CX3C present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CX3C are added to each well to "sandwich" the CX3C immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CX3C and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX3C concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16q13
Cellular Component: extracellular space; cell surface; plasma membrane; extracellular region; integral to membrane
Molecular Function: protein binding; chemokine activity; receptor binding
Biological Process: positive regulation of transforming growth factor-beta1 production; neutrophil chemotaxis; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; defense response; angiogenesis involved in wound healing; chemotaxis; leukocyte adhesive activation; macrophage chemotaxis; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of inflammatory response
Research Articles on FKN
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Product Notes
The Mouse FKN cx3cl1 (Catalog #AAA727496) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA727496 ELISA Kit recognizes Mouse FKN. It is sometimes possible for the material contained within the vial of "CX3C-chemokine/Fractalkine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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