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Typical Testing Data

Mouse Fractalkine ELISA Kit | FK/CX3CL1 elisa kit

Mouse Fractalkine ELISA Kit

Gene Names
CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
Reactivity
Mouse
Synonyms
Fractalkine; Mouse Fractalkine ELISA Kit; Fractalkine/CX3CL1/FKN/ABCD-3/C3Xkine/CXC3/CXC3C/NTN/NTT/SCYD1/FKN/Fractalkine/Neurotactin/ABCD-3/C3Xkine/chemokine (C-X3-C motif) ligand 1/CX3C membrane-anchored chemokine/CXC3/CXC3C/FKN/fractalkine/Neurotactin/neurotactin/neurotactin)/NTNsmall inducible cytokine subfamily D (Cys-X3-Cys); member 1 (fractalkine/NTTSmall-inducible cytokine D1/SCYD1C-X3-C motif chemokine 1/small inducible cytokine subfamily D (Cys-X3-Cys); member-1; FK/CX3CL1 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Sequence Length
397
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.391-25ng/ml
Sensitivity
0.234ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Testing Data

Typical Standard Curve

Typical Standard Curve
Related Product Information for FK/CX3CL1 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse FK antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse FK antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse FK amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse FK can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,203 Da
NCBI Official Full Name
fractalkine isoform 1
NCBI Official Synonym Full Names
chemokine (C-X3-C motif) ligand 1
NCBI Official Symbol
CX3CL1
NCBI Official Synonym Symbols
NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
NCBI Protein Information
fractalkine
UniProt Protein Name
Fractalkine
Protein Family
UniProt Gene Name
CX3CL1
UniProt Synonym Gene Names
FKN; NTT; SCYD1
UniProt Entry Name
X3CL1_HUMAN

Uniprot Description

CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: cell projection; cell surface; extracellular region; extracellular space; integral to membrane; perinuclear region of cytoplasm; plasma membrane

Molecular Function: CCR chemokine receptor binding; chemokine activity; protein binding; receptor binding

Biological Process: aging; angiogenesis involved in wound healing; cell adhesion; chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; leukocyte adhesive activation; leukocyte chemotaxis; lymphocyte chemotaxis; macrophage chemotaxis; microglial cell activation; monocyte chemotaxis; negative regulation of cytokine secretion; negative regulation of vasoconstriction; neutrophil chemotaxis; platelet activation; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; positive regulation of GTPase activity; positive regulation of inflammatory response; positive regulation of neuroblast proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of transforming growth factor-beta1 production; regulation of inhibitory postsynaptic membrane potential; response to hypoxia

Research Articles on FK/CX3CL1

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Product Notes

The Mouse FK/CX3CL1 cx3cl1 (Catalog #AAA764371) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA764371 ELISA Kit recognizes Mouse FK/CX3CL1. It is sometimes possible for the material contained within the vial of "Fractalkine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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